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Background in chemistry, biochemistry, molecular biology, virology, immunology, or related fieldKnowledge of assay development, process development, problem-solving, and statistical experimental designProtein optimization laboratory research experience preferred, particularly in recombinant protein expression.
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Experience in protein purification, protein analyses and mammalian cell culture is preferred. · Perform various scales of protein expression and purification using different technologies including affinity purification, ion exchange, size-exclusion, and ultracentrifugation.
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Protein purification process development to support our pre-pivotal purification & pilot operations group. Familiar with downstream purification process, which includes centrifugation, chromatography, and filtration.
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Serve as technical expert on downstream process development, including harvest and clarification; purification for target AAVs, removal of impurities and contaminating viruses, virus like particles, etc.
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Experience from an academic lab of purified proteins or from a CMO with small scale protein purification to support process development. Job Title: Biochemist II (Protein Purification.
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Please submit resumes with cover letter that specifically describes protein purification knowledge, equipment used, and techniques performed. Protein purification techniques, and assays.
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This work will require a number of biochemical and biologic techniques including (but not limited to): PCR; DNA and protein gel electrophoresis; Western blotting; standard cell culture; flow cytometry; transfections; DNA/RNA extractions; protein purification; buffer preparation; and microplate assays.
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O DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting. o Cloning, mapping and sequencing of DNA Recombinant virus production and purification.
$16.55 - $23.28 an hourFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Previous experience in small molecule drug discovery, specifically biochemical assay development and characterization of small molecule inhibitors and protein-ligand binding interactions.
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Experience Protein Purification for different expression systems using multiple techniques. Job Title: Scientist Protein Biochemist-Crystallographer. The successful candidate will integrate into an international and multidisciplinary team of scientists, engage in different research areas and work cross-functionally with the IO and Virology team to leverage crystallography to solve key molecular structures, produce high-quality proteins and contribute to the development and advancement of novel therapeutic strategies.
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Downstream process development experience and protein formulation experience for biologics would be a plus. Process development of lead molecules. Weve developed a proprietary AI-guided antibody discovery platform as a solid foundation for innovative biologics with diverse formats (e.g. mAb, bispecific antibody and fusion proteins), and other diverse modalities such as ADC, mRNA, and CART. Well-funded and supported by our unique research, development, and clinical validation ecosystem under our corporate umbrella, the team encompasses industry veterans with proven track records of bringing novel biologics to global clinical trials.
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Supports protein and peptide characterization, purification and conjugation workflows, as needed. Familiarity with basic techniques in protein and peptide characterization such as SDS-PAGE, isoelectric focusing, western blot, capillary electrophoresis, endotoxin quantification/removal, DSC, DLS, HPLC, SPR, BLI, etc.
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Preferred Qualifications: Experience with standard protein analysis i.e., ELISA, SDS-PAGE, western blot, CE-SDS (WES/JES), silver staining, and endotoxin testing. Key Responsibilities Include: Immuno Affinity Purification of rAAV serotypes from mammalian cells Empty/Full Capsid Separation via ion exchange chromatography and ultracentrifugation Develop and optimize production of high concentration and high quality AAV preps.
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The position will support experiments using DNA, RNA/transcriptomics, RT-PCR, protein/westerns and immunofluorescence/confocal approaches for molecular mechanistic and translational toxicology research.
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Experience with process development, technology transfer, process validation, and cGMP bio-manufacturing are highly desired. Experience designing and performing process development/characterization experiments, using either One Factor At a Time (OFAT) or Design of Experiments (DOE) with software like JMP or Minitab, etc., approaches.
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process development protein purification jobs Company: Abbvie in Not-specified, Alaska
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