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Role: seeking motivated, team-oriented individuals with expertise in protein purification process development to support our pre-pivotal purification & pilot operations group - an opportunity to work in teams for the early and late stage development of protein molecules.
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Incyte Corporation is seeking a motivated and experienced protein purification scientist to provide technical expertise in monoclonal antibody (including bispecifics) purification process development, characterization, and validation primarily for novel therapeutic monoclonal antibody products throughout all stages of clinical trials and commercialization.
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Perform protein purification methods on gravity or AKTA HPLC/ FPLC using affinity, ion exchange, hydrophobic, reverse-phase, and size exclusion chromatography. The main job role of this position is recombinant protein expression from culture platforms such as E. Coli and performing protein purification using various preparative chromatography, formulating the recombinant proteins according to existing or newly developed procedures.
$26.44 - $31.25 an hourFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Experience with basic protein purification and characterization techniques (i.e., protein A and nickel capture, HPLC, SDS-PAGE, Endotoxin testing, etc.) Participate and assist in the daily activities of the purification group such as antibody and antigen purification, and characterization by various techniques including protein concentration determination, CE-SDS, SDS-PAGE, various HPLC methods, and endotoxin measurement.
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Protein purification techniques: Experience in purifying recombinant proteins to high homogeneity for immunization purposes. Transient mammalian expression and protein purification.
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This role will be expected to independently execute cell culture process from harvest to final fill and to be cross trained in Downstream purification processes. Support protein material generation activities, including both mammalian cell culture and purification operations, from cell thaw to final formulated mAb.
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Experience with aseptic technique, microbial cultures, and protein purification. We have up to two openings for an Academic Graduate Appointee participating in developing novel approaches and platforms in support of synthetic biology research emphasizing cell-free protein production methodologies and vaccine development.
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Routine protein purification, including but not limited to:FPLC operation for routine FPLC purification processes (Affinity, SEC, CEX, AEX, HIC). Experience and core knowledge of protein production and purification is preferred.
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Top 3 Nice to Have Skill Sets: Protein purification, Protein characterization, working knowledge of AKTA Avant/PURE. Perform protein purification, QC, and protein bank registration to support the preclinical development of biopharmaceutical programs across multiple therapeutic areas.
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Expertise and knowledge in process development, scale up of formulation production and purification unit operations and tech transfer activities. The core platform technology comprises proprietary targeted lipid nanoparticles (tLNPs) that are composed of LNPs conjugated with a recombinant protein binder, such as a monoclonal antibody.
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Proven hands-on experience with multiple expression platforms (e.g., bacteria, insect cell, mammalian cell) and proficiency with AKTA instrumentation and a broad range of standard and unique purification and protein QC techniques are required.
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Title: Downstream Protein Purification-AKTA. Understand all aspects of purification process development, process scale up - TFF (Tangential flow filtration), Chromatography.
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Experience in protein purification, protein analyses and mammalian cell culture is preferred. · Perform various scales of protein expression and purification using different technologies including affinity purification, ion exchange, size-exclusion, and ultracentrifugation.
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Solid working knowledge of various manufacturing platforms (Microbial fermentation process, IB recovery and refolding, CHO cell culture and purification, Pegylation, PEGylated protein purification etc.
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Please submit resumes with cover letter that specifically describes protein purification knowledge, equipment used, and techniques performed. Protein purification techniques, and assays.
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process protein purification jobs Company: Abbvie in Not-specified, Alaska
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