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Experience with basic protein purification and characterization techniques (i.e., protein A and nickel capture, HPLC, SDS-PAGE, Endotoxin testing, etc.) Participate and assist in the daily activities of the purification group such as antibody and antigen purification, and characterization by various techniques including protein concentration determination, CE-SDS, SDS-PAGE, various HPLC methods, and endotoxin measurement.
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Solid working knowledge of various manufacturing platforms (Microbial fermentation process, IB recovery and refolding, CHO cell culture and purification, Pegylation, PEGylated protein purification etc.
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This work will require a number of biochemical and biologic techniques including (but not limited to): PCR; DNA and protein gel electrophoresis; Western blotting; standard cell culture; flow cytometry; transfections; DNA/RNA extractions; protein purification; buffer preparation; and microplate assays.
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Oligonucleotides to participate in the process design, development, and manufacture of RNAi therapeutic. (specializing in oligonucleotide synthesis, purification, and characterization.
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Interact with informatic engineers to develop processes that support automated antibody development workflows including antibody (DNA and protein sequence) registration, tracking, archiving, and inventory.
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5+ years of post-PhD experience in biotech industry, ideally in gene therapy or related next-generation drug development field such as biologics. Familiarity with protein engineering (involving model-guided approaches to protein design and molecular dynamic simulations.
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Oversee high-level strategic planning for developing bioinformatic analysis pipelines to assist pre-clinical therapeutic and platform teams, as well as to enable biomarker readouts for clinical trial development.
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Are excellent in most of the following: bacterial culturing, PCR, gel electrophoresis, molecular cloning, plate reader assays, cell-free expression systems, protein purification, buffer and media preparation, and DNA purification, sequencing, and analysis.
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Ability to perform bacterial protein expression and purification and the analysis of ligand/protein interactions by fluorescence, isothermal titration calorimetry, or thermostability assays.
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I deal Qualifications B.S. or M.S. in biology, bioengineering, or another relevant field 3+ years of experience in a biotechnology company Experience with common molecular biology techniques: PCR, nucleic acid purification, and protein purification Experience with medium and high-throughput molecular biology workflows is a plus Experience with cell culture, microbes (e coli or yeast), or phage A working understanding of next-generation sequencing workflows.
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Responsibilities -Develop assays for GMP release of gene therapy products-Develop assays for product characterization of gene therapy products-Develop assays for AAV/protein/DNA to assist in evaluation of process change, formulation andstability study-Report project status (development plans, timelines, and results) to supervisor and technical teams.
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Coordinate protein expression and purification with other teams at the firm. At least 2 years of research experience in protein biochemistry, protein engineering or related field.
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Coordinates with Cancer Immunology, Protein Chemistry, Antibody Engineering, and Translational Oncology groups to advance lead molecules of the projects. Supports biochemical and cellular characterization of protein therapeutics for immuno-oncology.
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Some background in protein engineering, immunology, virology, and/or assay development. Strong background in protein engineering, immunology, virology, and/or assay development.
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As a Pharmacokinetics Scientist, you will utilize your experience to further characterize the novel kinetics of our new therapeutic modality and build complex models that inform study designs and decision-making throughout the drug development process.
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process development protein purification jobs Company: Abbvie in San Francisco, CA
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