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Utilize basic molecular biological techniques (, designing gene constructs, cloning, PCR, Western blot, gene transfection, mutagenesis, plasmid amplification, cell culture). The Department of Molecular and Cellular Biology at the University of Arizona is looking for a candidate to work with the Prof. Ingmar Riedel-Kruse Lab as an Assistant Staff Scientist.
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Conduct a wide range of biochemistry, molecular and cellular biology techniques to express, purify and characterize drug candidates (e.g. DNA/RNA isolation, plasmid construction, PCR, Octet, HPLC, mammalian cell culture, transient and stable transfections.
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The candidate should have excellent writing and communication skills and be familiar with techniques such as multi-color flow cytometry, cell isolation, tissue culture, ELISA, qRT-PCR, RNAseq analysis, and animal handling.
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Perform in vitro assays to evaluate efficacy of drug candidates (e.g. ELISA, flow cytometry, cell proliferation, anti-microbial assays). Perform in vitro assays to evaluate efficacy of drug candidates (e.g. ELISA, flow cytometry, cell proliferation, anti-microbial assays.
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Experience with complex cellular immunology techniques, such as flow cytometry, ELISA/ELISPOT, tissue processing, cell, bacterial, vial and/or parasitic culture, sterile technique, DNA/RNA extractions and PCR.
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This project will include cell culture and molecular biology techniques, microscopy, data collection, data analysis/processing, presentations at national meetings, and contribution to written publications.
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Perform cell culture techniques related to cell-based assays such as antibody-dependent cellular cytotoxicity (ADCC,) microneutralization, Median Tissue Culture Infectious Dose (TCID50) assays would be preferred.
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This position additionally requires providing technical support for in vitro and in vivo experiments, encompassing molecular biology, cell culture, and flow cytometry, for the benefit of post-doctoral fellows and graduate students within the laboratory.
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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Expertise in mammalian cell culture, flow cytometry, FACS sorting, protein characterization and protein display technologies, RASyn’s platform integrates computational protein design and engineering, machine learning, synthetic biology, and single cell sequencing and will enable cheaper, faster, and larger scale production and optimization of biologics, enzymes, and biomolecule libraries.
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This will include but is not limited to Western blots, RT-PCR, immunohistochemistry, ELISAs, cell culture, etc. The Department of Integrative Physiology and Metabolism is dedicated to the discovery, development, and promotion of biomedical knowledge involving basic and applied biomedical sciences with an emphasis on the study of cellular and molecular basis of tissue function.
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Hands-on experience with fluorescence and confocal imaging, biochemistry, cellular immunology, molecular biology, cell culture, flow cytometry, gene expression, quantitative analysis, single cell RNAseq/ATACseq/bioinformatics analysis.
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Provide technical support on multi-disciplinary collaborative research projects under a central services model and develop and apply new techniques applicable to stem/progenitor cell culture and regenerative medicine research.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Our non-profit Client is looking for a Cell Engineering lab Tech/ Research Associate to focus on utilizing cutting-edge CRISPR gene editing methods to support cell engineering projects that are part of a larger research platform designed to study disease biology at the cellular level and identify new therapeutic modalities.
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