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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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The scientist will be proficient in multiple cell biology assays that could include Western blotting, flow cytometry, siRNA/shRNA KD assays, ELISAs, cell proliferation, cellular cytotoxicity, immune cell isolation from whole blood, capillary protein electrophoresis, cell culture, transfection/infection, immunoprecipitation.
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The specialist will be involved in projects that utilize cellular culture models and next-generation sequencing that carry a high potential to ultimately advance cancer care. The Section of Pediatric Hematology/Oncology provides care for patients and families with acute and chronic medical conditions, or other hematologic diseases, requiring the specialty knowledge and skills of Hematology, Oncology and Stem Cell Transplantation.
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Procedures include but are not limited to: isolation and analysis of DNA, RNA, and protein; cell culture; and mouse handling and experimentation. Assisting with molecular biology techniques such as cloning and plasmid purification, as well as isolation and analysis of RNA, DNA, and protein from mouse tissue and cellular samples.
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Knowledge of cellular, molecular, and biochemical assays to characterize stem cell culture (Flow cytometry, immunofluorescence, imaging assays, microscopy). Strong fundamental knowledge of cell biology, human embryonic stem cell culture, and stem cell research.
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Duties in San Francisco will include performance of cellular immunology assays, mostly based on flow cytometry (Cytek Aurora spectral flow cytometer, FACS ARIA cell sorter, and LSRII cytometers) or ELISpot, development and validation of new flow panels and assays, data management, as well as using software to analyze high parameter flow data.
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Experience with bacterial cell culture, molecular or biochemical assays (PCR, ELISA) and protein purification. 1-2 years academic or industrial experience maintaining cell lines and performing mammalian cell culture.
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Demonstrated proficiency in techniques across multiple fields, including molecular and cellular biology (DNA/RNA isolation, PCRs, gene expression, immunostaining, protein blotting, cell culture, developing disease models in mice, etc.
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Proficient knowledge of LC-MS, enzyme kinetics measurements, and/or cell culture is highly desirable. We are looking for a highly motivated Scientist to develop and conduct biochemical and/or cellular assays in the field of in vitro ADME to evaluate potential therapeutics.
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Mammalian cell line engineering, mammalian cell culture, molecular biology, flow cytometry, PCR, bench, project management. Transfection, mammalian cell culture, cloning and screening.
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The Executive Director of Cell Therapy Vector Operations will report to the Vice President of CTTO. This role is a key leadership position within the BMS CTDO Division as we prepare to commercialize autologous cellular biologics to overcome tumor evasion and potentially eradicating cancer cells.
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Proven experience in laboratory cellular biology techniques applied to development of physiologically relevant cellular assays to evaluate biological mechanisms (including but not restricted to primary cell culture, flow cytometry and ELISAs.
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Experience in cell handling (tissue culture, FACS), tissue/cellular imaging preferred. Preference will be given to candidates with expertise in one or more of the following areas: live cell imaging, cell/tissue engineering, molecular biology techniques and/or implant surface materials fabrication.
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The Research Associate I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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In vitro work includes a range of cellular assays, cell culture including multiparametric flow cytometry and molecular biology techniques to help interpret in vivo observations. Invitro cell culture.
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