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We combine (1) the development of multi-modal single-cell and single-molecule genomics technologies, (2) the generation of cellular atlases of healthy and diseased tissues using single-cell methods, (3) organoid-based cell culture systems to model healthy and diseased states ex vivo (4) and CRISPR-based genetic perturbations for high-throughput functional screens of regulatory features.
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Experience working with mammalian cell culture, and multi-color flow cytometry. Experience with standard laboratory molecular biology software packages, RNA biology, in vivo gene delivery, mouse primary immune cell culture.
$40 - $65 an hourInternExpandApply NowActive JobUpdated 9 days ago - UpvoteDownvoteShare Job
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Demonstrated experience of 3-5 years in executing a variety of cell biology techniques, including mammalian cell culture, in vitro cellular assays (e.g., cell viability, proliferation, invasion, apoptosis), and luciferase assay.
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Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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The position involves mammalian cell culture, flow cytometry, molecular biology (plasmid construction, CRISPR gene editing), viral transduction to generate gene modified immune effector cells.
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Sterile tissue culture techniques, cell-based imaging and assays (immunohistochemistry, fluorescence microscopy, confocal microscopy, ELISA) and mesenchymal stem cell biology experience are also preferred.
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Continuous internalization and utilization of a broad range of molecular biology, genome engineering, cell culture, and screening techniques to identify, validate, and optimize therapeutics.
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Utilize basic molecular biological techniques (, designing gene constructs, cloning, PCR, Western blot, gene transfection, mutagenesis, plasmid amplification, cell culture). The Department of Molecular and Cellular Biology at the University of Arizona is looking for a candidate to work with the Prof. Ingmar Riedel-Kruse Lab as an Assistant Staff Scientist.
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The candidate should have excellent writing and communication skills and be familiar with techniques such as multi-color flow cytometry, cell isolation, tissue culture, ELISA, qRT-PCR, RNAseq analysis, and animal handling.
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Advanced understanding of primary cell culture and experience with equipment such as CliniMACS Plus, CliniMACS Prodigy, LOVO, bioreactors (single-use and fixed-bed), chromatography skids, TFF (hollow-fiber and flat-sheet) and WAVE bioreactors such as Xuri, G-Rex system.
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Conduct a wide range of biochemistry, molecular and cellular biology techniques to express, purify and characterize drug candidates (e.g. DNA/RNA isolation, plasmid construction, PCR, Octet, HPLC, mammalian cell culture, transient and stable transfections.
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Applicants must be US Citizens and be proficient in cell culture, flow cytometry, protein analysis, molecular biology, and be comfortable with small animal polytrauma and microsurgical techniques.
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Perform in vitro assays to evaluate efficacy of drug candidates (e.g. ELISA, flow cytometry, cell proliferation, anti-microbial assays). Perform in vitro assays to evaluate efficacy of drug candidates (e.g. ELISA, flow cytometry, cell proliferation, anti-microbial assays.
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Working knowledge on protein purification and hands-on experience on chromatography workstations preferred. The ideal candidate will function in a cutting-edge laboratory environment as part of a multi-disciplinary team of protein engineers, immunologists, molecular biologists, and protein biochemists to discover novel therapeutics with opportunity to develop cGMP or GLP compliant skills in an industrial laboratory setting.
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The candidate is expected to have basic immunology training and strong cellular immunology and cell culture technical skills. Experienced in performing cellular immunological techniques, including multi-color flow cytometry, ELISA, MSD and in vitro immune cell assays.
$107,652 - $131,899 a yearFull-timeExpandApply NowActive JobUpdated 13 days ago
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