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Design and conduct in vitro cellular assays (e.g. mammalian cell culture, Lentiviral Transduction, 2D and 3D cell culture, SDS-PAGE/Western blot, qPCR, CRISPR-based gene editing) and implement relevant assay readout technologies (e.g. Luciferase, CellTiter-Glo, TR-FRET/BRET, MSD.
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REQUIRED SKILLS : Bioanalytical, BL2, Cell Culture, Cytotoxicity, Good Documentation Practices (GDP), Good Manufacturing Practices (GMP),DDITIONAL SKILLS: Laboratory, Maintenance, Method Validation, GMP, QA, Radioactive, SOPs, Validation Protocols, Analytical Assays, Bioassay, Biochemistry, Cellular, Cellular Biology, Immunology, Operations, Good Documentation Practices.
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Mammalian cell line engineering, mammalian cell culture, molecular biology, flow cytometry, PCR, bench, project management. Transfection, mammalian cell culture, cloning and screening.
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Duties in San Francisco will include performance of cellular immunology assays, mostly based on flow cytometry (Cytek Aurora spectral flow cytometer, FACS ARIA cell sorter, and LSRII cytometers) or ELISpot, development and validation of new flow panels and assays, data management, as well as using software to analyze high parameter flow data.
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Micro- Aseptic techniques, cell culture, cell plating and staining / Molecular- Assays, blotting, PCR, imaging). Strong knowledge and hands-on experience in cell biology and biochemical assays such as cell culture, ELISA and Western-blot.
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We use a broad range of techniques from high resolution imaging, bioinformatics, cell biology and biochemical assays, viral-mediated gene delivery, functional and molecular studies in vivo and in vitro, using tissue culture and mouse models.
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Primary responsibilities that include demonstrating proficiency to perform clinical release testing on cell therapy products, including but not limited to, immunophenotyping (Flow Cytometry), sterility, mycoplasma, viability, residual bead counting, and ex vivo culture assays.
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Working under the general supervision of a Senior Technical Research Assistant, Manager or Principal Investigator and following established policies and procedures, conducts a variety of specialized experimental procedures in human iPS cell culture and differentiation, Human fibroblast culture, fluorescence microscopy, FACS analysis, cell biology assays and molecular biology techniques.
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Experience with multi-color flow cytometry, T cell functional assays, ELISA assays, culture of mammalian cell lines and primary immune cells is required. Experience with dendritic cell differentiation and culture is a plus.
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Cell culture techniques Standard cell and molecular biology techniques, such as DNA and RNA purification, cloning, PCR, western blotting Ability to write routine reports and correspondence.
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Utilizes professional knowledge of: theories, principles, methods, and techniques of immunology, cell biology, and microbial pathogenesis, such as ELISA, ASC assays, cellular proliferation assays, western blotting, cell culture, small animal handling (such as mice or guinea pigs), and aseptic technique.
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Must have training relevant to cell therapy, stem cells, cell culture, flow cytometry and cryogenicsProvides scientific consulting services to CSCC customers, clients and patientsResponds to any facility alarms 24 hours a dayOther duties as assigned.
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Assist in the evaluation of Shigella vaccines in clinical and pre-clinical studies to include immunizing small animals, collecting blood, lymph nodes mucosal washes, and determining the immune response elicited after vaccination using cellular proliferation, antibody secreting cell (ASC), ELISA, and immunobead multiplexing assays.
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Successful postdoctoral scholars would require experience and skills that would include mammalian cell culture techniques, establishing cancer chemotherapy resistance cell lines, develop siRNA and chemotherapy drug delivery systems in cancer cell lines and in vivo mice models, molecular and cell biology techniques, cellular assay development, biochemistry and mice handling.
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We combine (1) the development of multi-modal single-cell and single-molecule genomics technologies, (2) the generation of cellular atlases of healthy and diseased tissues using single-cell methods, (3) organoid-based cell culture systems to model healthy and diseased states ex vivo (4) and CRISPR-based genetic perturbations for high-throughput functional screens of regulatory features.
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