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Mammalian cell line engineering, mammalian cell culture, molecular biology, flow cytometry, PCR, bench, project management. Flow cytometry, PCR, and other related molecular and cellular biology techniques.
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As a senior-level molecular/cellular biologist, you will help us disrupt biologics protein and virus vector production. We seek an experienced independent molecular/cellular biologist or virologist motivated to develop our technology further toward full commercialization.
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Design and conduct in vitro cellular assays (e.g. mammalian cell culture, Lentiviral Transduction, 2D and 3D cell culture, SDS-PAGE/Western blot, qPCR, CRISPR-based gene editing) and implement relevant assay readout technologies (e.g. Luciferase, CellTiter-Glo, TR-FRET/BRET, MSD.
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Knowledge/experience performing sterile tissue culture and cellular/immunology/molecular techniques. Duties in San Francisco will include performance of cellular immunology assays, mostly based on flow cytometry (Cytek Aurora spectral flow cytometer, FACS ARIA cell sorter, and LSRII cytometers) or ELISpot, development and validation of new flow panels and assays, data management, as well as using software to analyze high parameter flow data.
ExpandApply NowActive JobUpdated 11 days ago - UpvoteDownvoteShare Job
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We use a broad range of techniques from high resolution imaging, bioinformatics, cell biology and biochemical assays, viral-mediated gene delivery, functional and molecular studies in vivo and in vitro, using tissue culture and mouse models.
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Proficiency with tissue culture techniques, including cell culture (cell line and primary cells) and cell transfection using lipofection and lipid nanoparticles. Addition Therapeutics is currently seeking a Senior Research Associate with extensive cellular and molecular biology experience.
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We are seeking a highly motivated and talented scientist with background in cellular and molecular biology and assay development to join the Biotherapeutics and Genetic Medicine organization to support in vitro pharmacology work related to Genetic medicine research on AAV.
ExpandApply NowActive JobUpdated 16 days ago - UpvoteDownvoteShare Job
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Frequently used techniques are multicolour flow cytometry, cell culture, functional cellular assays, life cell imaging, and immunoassays. flow cytometry, cell culture/functional cellular assays, and.
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Working under the general supervision of a Senior Technical Research Assistant, Manager or Principal Investigator and following established policies and procedures, conducts a variety of specialized experimental procedures in human iPS cell culture and differentiation, Human fibroblast culture, fluorescence microscopy, FACS analysis, cell biology assays and molecular biology techniques.
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At least 2 years postgraduate, hands-on experience in mammalian cell culture and protein biochemistry such as SDS-PAGE, ELISA and affinity chromatography are required. The Research Associate will be responsible for conducting hands-on experiments including but not limited to molecular biology, mammalian cell culture, recombinant protein purification and characterization.
ExpandApply NowActive JobUpdated 11 days ago - UpvoteDownvoteShare Job
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Sr Scientist I: BS in molecular biology, biochemistry, pharmacology, or a related field with typically 10+ years of experience, MS and typically 8+ years of experience, or PhD and typically 0+ years of experience, preferably in the areas of cellular assay development and screening.
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Developing and optimizing biochemical, cellular, and molecular methods as needed. Operating from state-of-the-art labs in South San Francisco, our team of experienced scientists and drug developers is leading scientific breakthroughs while cultivating a vibrant, dynamic culture committed to profoundly impacting patient lives.
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Molecular biology (cloning, restriction digests) under the supervision of a senior scientist Protein science, including western blot Cell culture, sterile technique, and transfection. Skills such as, histology, cloning, general mammalian cell culture, transfection and familiarity with in vivo rodent models will be considered advantageous.
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3-5 years of experience performing cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay. Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
ExpandApply NowActive JobUpdated 12 days ago
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