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Proficiency with tissue culture techniques, including cell culture (cell line and primary cells) and cell transfection using lipofection and lipid nanoparticles. · Proficiency with standard cell biology techniques, including but not limited to fluorescence microscopy, flow cytometry, and ELISA.
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Frequently used techniques are multicolour flow cytometry, cell culture, functional cellular assays, life cell imaging, and immunoassays. flow cytometry, cell culture/functional cellular assays, and.
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Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.
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Translational studies involving archival and fresh human tumor biopsied tissues and cell lines, and establishment of preclinical cell lines (including 3D spheroid culture), animal models using patient-derived xenografts (PDX) method.
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Experience with bacterial cell culture, molecular or biochemical assays (PCR, ELISA) and protein purification. 1-2 years academic or industrial experience maintaining cell lines and performing mammalian cell culture.
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Your technical expertise as a Research Analyst III in the mammalian cell culture will contribute to our client's innovative therapies which will impact the quality and duration of life. Significant mammalian cell culture expertise: maintenance, freezing, thawing, and passaging.
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Successful postdoctoral scholars would require experience and skills that would include mammalian cell culture techniques, establishing cancer chemotherapy resistance cell lines, develop siRNA and chemotherapy drug delivery systems in cancer cell lines and in vivo mice models, molecular and cell biology techniques, cellular assay development, biochemistry and mice handling.
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The successful candidate will play a key role in advancing our understanding of plant cell culture processes, with a focus on molecular and cellular biology, genetics, and plant cell culture fermentation.
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Working under the general supervision of a Senior Technical Research Assistant, Manager or Principal Investigator and following established policies and procedures, conducts a variety of specialized experimental procedures in human iPS cell culture and differentiation, Human fibroblast culture, fluorescence microscopy, FACS analysis, cell biology assays and molecular biology techniques.
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The primary responsibilities of the RAs will include isolation and culture of primary human immune cells, functional analysis of engineered cells via high throughput flow cytometry, assistance with genomic techniques such as single-cell RNA sequencing and CRISPR gene editing, among others.
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Basic knowledge of cell culture manufacturing principles, risk assessments (FMEA), Chemistry, Manufacturing, and Controls (CMC) principles, and tech transfer. Experience with PSC cell culture, expansion, banking, and differentiation is a must.
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In this critical role you will p erform laboratory work in a cGMP manufacturing setting, primarily working with iPSC cell culture and differentiation, following bio-manufacturing procedures and manufacturing schedules to facilitate on-time delivery of high-quality finished products.
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Specifically, the applicant should have experience and skills in Western assay, polymerase chain reaction (PCR), recombinant DNA manipulation, CRISPR, cell transfection, cell culture, immunohistochemistry, and fluorescence microscopy.
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Next generation sequencing (NGS) sample preparation, cell culture, flow cytometry. This key position will manage and conduct research and development projects related to 1) genomics investigations of cancer samples and development of novel genomics methodologies for oncology diagnostics and 2) tumor immunology projects focused on anti-neoantigen T cell responses with an aim towards the development of personalized cellular immunotherapies.
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Experience in cell line development, ELISA, PBMC biomarker, cell culture, cellular assays, gene expression is desirable. Perform cell biology and molecular biology studies to support early drug discovery projects.
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