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Specific Responsibilities:Molecular Biology:DNA, RNA, Protein isolation, purification and expression Western blottingPCR, RT-PCRPlasmid preparationCloning, mapping and sequencing of DNA Recombinant virus production and purificationCellular Biology:Cell culture, cell isolation Functional studies TransfectionImmunologyAntibody production and purificationAdministrationMaintains detailed records of results which may include collecting, extracting & entering data.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linked immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Familiar with basic assays such as tissue culture, cell lines and tissue, analyzing protein and RNA signaling assays (western blot, qPCR, etc.) UPMC Magee-Womens Hospital is seeking a Full Time Senior Research Assistant, BS to support the Gynecological Oncology lab.
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ResponsibilitiesExtract protein, DNA and/or RNA from necropsied animal tissueRun ddPCR, qPCR, ELISA, Western Blot etc analysis on tissue extractsCell culture, Neutralizing antibody assays and total antibody assaysSample analysis assay optimizationSOP writingData recording and analysisQC analysis This position directly impacts preclinical outcomes including proof of concept, ED50, MED, clinical hold response and IND enabling studies.
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Molecular techniques including transfections, cloning, DNA/RNA extraction and PCR, restriction enzyme digestion. The Research Scientist is responsible for support within the Construct Team. Must have a strong background in molecular and immunological assays, protein production and optimization, and bioinformatics.
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Support general lab activities such as: making solutions and buffers as needed and performing basic molecular cloning (primer design, PCR, DNA/RNA purification), as well as performing protein expression and protein purification and other molecular biology techniques.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linker immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Analyzes DNA/RNA/Protein by PCR, Northern Blot, and Western Blot (15%) Upon request, performs other tasks requested by faculty to maintain a smooth operation of the laboratory (5%). Prepares daily records of experimental procedures and results maintain laboratory supplies Extracts DNA/RNA/Protein from mammalian cells.
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Experience performing DNA, RNA, and protein preparation and purification is highly desired. Experience performing qPCR, qRT-PCR, DNA sequencing, ChIP-seq, mRNA isolation, northern blots, cDNA synthesis, immunoblots, co-immunoprecipitation, immunohistochemistry, and in situ hybridizations, are highly desired.
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Requires experience in tissue culture, DNA & RNA preparation, PCR, DNA cloning, protein synthesis and electrophoresis. Experience in molecular techniques involving plasmid DNA preparations and transfection, the analysis of gene expression including QPCR, microarrays, chromatin immunoprecipitation, evaluation of protein biochemistry with 2D gels, animal models, the yeast-two-hybrid system, confocal microscopy and tissue culture are also preferred.
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DESCRIPTIONUnder the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of cellular protein quality control by carrying out various biochemistry, cell biology and molecular biological procedures in mammalian cells and Zebrafish.
$21.39 - $23.06 an hourFull-timeExpandApply NowActive JobUpdated 5 days ago - UpvoteDownvoteShare Job
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Performs basic and advanced molecular and cellular biology techniques including: PCR, tissue culture, DNA/RNA/Protein extraction, and processing tissue for immunotyping. Performs basic and advanced molecular and cellular biology techniques including: PCR, tissue culture, DNA/RNA/Protein extraction, and processing tissue for immunotyping.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry) · Experience with high-throughput drug screening is a plus · Possess some trouble shooting and problem-solving skills to resolve technical questions in a multi- disciplinary environment.
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Additional duties include but are not limited to: hyperpolarized NMR spectroscopy and imaging, cell culture, DNA preparation, RNA isolation, protein purification and preparation, PCR-based genotyping, RT-PCR, Western Blotting, molecular subcloning, flow cytometry, immunofluorescence and live cell imaging.
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