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Measurement of molecules in vitro: Electrophoretic mobility shift assay (EMSA) for protein-DNA or protein-RNA interactions, Co-immunoprecipitation to detect protein-protein or protein-RNA interactions.
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Biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry) transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and.
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Perform experiments involving PCR, Quantitative PCR, Western Blots, PCR Arrays, RNA and DNA extraction, protein expression, and purification. The incumbent will perform experiments involving PCR, Real-time PCR, Northern and Western Blots, PCR Arrays, RNA and DNA extraction, protein expression, and purification.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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An ideal candidate will have AAV purification experience, or broad recombinant protein and vector production experience, a collaborative spirit and a desire to work in a fast-paced startup environment.
$82,000 - $124,000 a yearFull-timeExpandApply NowActive JobUpdated 1 days ago - UpvoteDownvoteShare Job
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Knowledge of two-hybrid assay screening, focusing on studying molecular interactions, such as protein-protein, protein-DNA, and protein-RNA interactions 6. Knowledge of protein/antibody discovery and optimization using in vitro display (such as phage or yeast) technology 5.
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QPCR using the Life Technologies QS5 systemMolecular Biology DNA, RNA, and protein purification and quantification, gel electrophoresis, molecular cloning, site-directed mutagenesis, and recombinant protein expression.
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Demonstrated experience with standard molecular biology techniques (DNA/RNA/protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence) Perform DNA, RNA, and protein isolation from mammalian cells.
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Molecular Biology DNA, RNA, and protein purification and quantification, gel electrophoresis, molecular cloning, site-directed mutagenesis, and recombinant protein expression. Molecular Biology DNA, RNA, and protein purification and quantification, gel electrophoresis, molecular cloning, site-directed mutagenesis, and recombinant protein expression.
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Conduct a wide range of biochemistry, molecular and cellular biology techniques to express, purify and characterize drug candidates (e.g. DNA/RNA isolation, plasmid construction, PCR, Octet, HPLC, mammalian cell culture, transient and stable transfections.
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The incumbent will be responsible for drug screening, cell-based assay development, and biomarker discovery. Work at the bench to develop and execute established and novel molecular and cell-based assays for drug screening as well as understanding molecular pathways (Proliferation, Apoptosis, Flow Cytometry etc.
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Skills in isolation of biological materials, (DNA, RNA and protein) from tissues and cell lines, as well as the cataloging and management of resources using relational computer databases.
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Prior experience with protein and/or RNA engineering. Proven hands-on experience with molecular biology techniques: Gibson assembly, Golden Gate assembly, DNA & RNA isolation and plate-based PCR assays (qRT, ddPCR, etc.
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This position will be part of a highly matrixed, cross-functional discovery team with opportunities to interact across protein engineering, plant pathology and analytical chemistry functional teams.
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Proven hands-on experience and knowledge with routine molecular biology techniques (DNA/RNA/protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence.
$96,000 - $128,000 a yearExpandApply NowActive JobUpdated 1 days ago
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