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GentiBio is seeking a collaborative and motivated Associate Scientist I/II Contractor to join our Discovery team in Cambridge, MA. The successful candidate will design, optimize and execute in vivo studies to characterize engineered T regulatory (EngTreg) cells to support our early-stage programs.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Experience in flow cytometry, including sorting of cells based on fluorescent labels. Proficiency in flow cytometry analysis and quantification (ie FlowJo) Your daily responsibilities include the collection of in vitro and in vivo samples, and library preparation for genomic assays, including single cell genomics, CUT&TAG, CUT&RUN, WGS, and Amplicon-sequencing.
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Support in vitro, in/ex vivo multiparametric assays including high parameter flow cytometry, histology, mutliplex immunofluorescence, high content imaging, and biochemical assays. Experience with high-parameter flow cytometry, immunohistochemistry & immunofluorescence.
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Familiarity with IND-enabling studies and preparing materials for the pharmacology section of an IND.Excellent skills with cell culture such as primary cell culture, stable and transient protein expression, fixed and live cell imaging by IHC or immunofluorescence, flow cytometry.
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You have experience with primary human immune cell activation assays using multparameter flow cytometry. You have experience with T cell cytotoxicity assays using endpoint flow cytometry measurements.
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Expertise on multi-color flow cytometry assays from ex vivo tissue or cells. Expertise in multi-color flow cytometry. Work with in vivo colleagues with experiments in support of Inflammation & Immunology therapeutic portfolio.
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Experience in flow cytometry, microbiology, PCR and ELISA testing is a plus. Demonstrate basic knowledge of the GXP cellular therapy product manufacturing, and program development and evaluation, as well as needs assessment and marketing of the programWorking knowledge of flow cytometry, molecular biology, and genetic engineering is a plus.
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Hands-on experience in multicolor flow cytometry and other routine immunological assays. The position involves collaboration with protein science, in-vivo biology, single-cell sequencing, bioinformatics, translational and clinical teams to execute and advance HiFiBiO's drug discovery and translational research.
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The CNS Research Group at Alnylam seeks an Associate Scientist II with expertise in transgenic vivo models of disease. The Associate Scientist II will actively participate in a vibrant, multidisciplinary drug discovery team and use novel, innovative tools and technologies to investigate genetically validated targets amenable to RNAi Therapeutics.
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Developing and optimizing project specific mechanistically relevant short term pathway activation as well as disease mouse models of disease - subsequent analysis of PK/PD and associated disease-related pathologies by various techniques such as flow cytometry, histology, protein multiplex ELISAs and focused or full transcriptome / proteome analysis.
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The ideal candidate will have neuroscience research experience employing a variety of in vitro/in vivo electrophysiology techniques including traditional whole-cell patch-clamp, extracellular field potential recordings, and qEEG. Additional computational or programming experience is also required.
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Positions are focused early career including the ranks of: Postdoctoral Research Associate or Associate Research Scientist. At least 2 years of postdoctoral experience is required for the associate research scientist position.
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Hands-on experience in tissue culture, plate-based assays, RNA isolation, gene expression analysis (qPCR), western blot, ELISA, multiplex protein analysis, flow cytometry preferred. Experience with hands on and/or managing in vivo work at CROs is a plus.
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Proven experience working with complex matrices and cell isolation, ideally also exposure to technologies required for cell and gene therapy products such as NGS, PCR variations including ddPCR, flow cytometry.
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vivo flow cytometry associate scientist jobs in Cambridge, MA
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