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Extensive hands-on experience with standard immunological techniques including multi-parameter flow cytometry, cell sorting, immunoassays such as ELISA, Cytotoxicity, in vitro co-culture assays (MLR, PBMC cultures, etc), and reporter bioassay development.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Expertise in cell culture, PCR, ELISA, western blot, and flow cytometry. The successful candidate will join a dynamic, multi-disciplinary team and play a key role in developing and applying in vivo models to evaluate novel cancer therapies in the early-stage drug discovery pipeline.
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Expertise in at least one of the following assay methods: ELISA, Western blot, qPCR, flow cytometry, ChIP. Implement target-specific assays to assess target and drug activity in vitro and/or in vivo, as appropriate.
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Develop and validate immunological assays (, flow cytometry, ELISA, CyTOF, cytokine profiling) to evaluate drug candidates' efficacy and mechanism of action. Learn more about Flagship at Position Overview:We are seeking a highly skilled PhD level Principal Scientist with proven experience in immunology and preclinical research to join our team.
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Proficiency in in vitro and in vivo immunological techniques including ELISA, flow cytometry, and cellular assays. Design, execute, analyze, and document in vivo and in vitro experiments within the Rilzabrutinib project team.
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Cambridge, MA. Internal Title: Principal Scientist I/II About the role: The successful candidate will join a dynamic, multi-disciplinary team and play a key role in developing and applying in vivo models to evaluate novel cancer therapies in the early-stage drug discovery pipeline.
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Experience with some – but not necessarily all – of the following techniques: PCR, molecular cloning, viral transduction, gene editing, immunoblotting, cell culture, colony-formation assays, ex vivo and in vivo modeling of cancer, immunohistochemistry, flow cytometry, fluorescence-activated cell sorting, and next-generation sequencing library preparation.
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Proficiency in primary immune cell isolation and stimulation, flow cytometry, proliferation and apoptosis assays, and immunoassay techniques/platforms (e.g. Western, ELISA, Mesoscale) to measure signaling intermediates and cytokines.
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Cambridge, MA.Internal Title: Principal Scientist I/II#LI-OnsiteAbout the role:The successful candidate will join a dynamic, multi-disciplinary team and play a key role in developing and applying in vivo models to evaluate novel cancer therapies in the early-stage drug discovery pipeline.
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Execute in vitro and ex vivo nonclinical experiments for the development of genetic medicines for the inner ear, including cell culture transfection/transduction, cochlear explant generation and analysis, and various molecular readouts such as flow cytometry, ddPCR/qPCR, and western blot.
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Hands-on experience in multicolor flow cytometry and other routine immunological assays. The position involves collaboration with protein science, in-vivo biology, single-cell sequencing, bioinformatics, translational and clinical teams to execute and advance HiFiBiO's drug discovery and translational research.
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Support in vivo studies with molecular analysis of cochlear tissue for DNA, RNA, and protein readouts. The scope of work includes the design, execution, and analysis of in vitro and ex vivo experiments using novel AAV vectors.
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Demonstrated experience in diverse molecular and biochemical laboratory techniques applied to cell characterization at molecular or genetic levels (immunofluorescence, flow cytometry, qPCR, RNA sequencing)Well organized, self-motivated, team oriented, ability to multitask with attention to detailExcellent communication and writing skillsProficient in computational analysis and coding pipelines (R Studio, Python, etc.
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Proficient in performing laboratory work related but not limited to tissue culture, qPCR, western blot, multi-parametric flow cytometry, immune cell culture using activation/expansion/exhaustion assays, proliferation and tumor killing assays, 2D/3D co-culture activity assays, ELISA, Luminex, processing of DNA/RNA/protein.
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vivo flow cytometry scientist jobs in Cambridge, MA
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