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Proficiency in a variety of bioanalytical techniques including, but not limited to, ELISA, MSD, HTRF, flow cytometry, qPCR, NGS, mass spectrometry, and cellular imaging techniques. Technical expertise with in vitro and ex vivo assays, mammalian tissue culture, reporter cell lines (including custom generation of reporter systems), and primary human cell cultures.
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Reporting to the Principal Scientist, you will work closely with our team using both in vitro cellular systems and in vivo humanized mouse models to optimize oligonucleotide delivery. Support in vitro and in vivo validation of brain shuttles, using protein and cell binding assays, and in vivo brain and blood pharmacokinetics and pharmacodynamic (PK/PD) studies in mouse models.
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Proficiency with the culture of primary cells, in vitro assays, flow cytometry and analysis, and in vivo immunological models. Developing and implementing in vivo/ex vivo drug delivery and functional studies.
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Expertise in in vivo mouse models, in flow cytometry, multiplex cytokine read-outs, qPCR is essential. Experience in molecular and cellular biology techniques including RT-PCR, ELISA, flow cytometry and assay development.
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Assays, flow cytometry and analysis, and. in vivo/ex vivo. assays, flow cytometry and analysis, and. Knowledge of computational/modeling systems (MHC/TCR predictors, RNseq data analysis, ect.
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Designing and performing a variety of laboratory assays and procedures that encompass molecular biology, biochemistry, cell culture, flow cytometry, immunohistochemistry (including tissue processing and cryosectioning), and FluorescenceIn Situ Hybridization techniques.
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Perform relevant in-vitro and in-vivo work to evaluate efficacy of therapeutic nanoparticles. Set-up, develop and prepare routine and specialized theranostic nanoparticles for in-vitro and in-vivo experiments.
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Collaborate with Protein Sciences to optimize the developability profiles of candidate molecules, and with In Vivo Pharmacology team to evaluate PK/PD, efficacy, and toxicity. Excellent skills with cell culture such as primary cell culture, stable and transient protein expression, fixed and live cell imaging by IHC or immunofluorescence, flow cytometry.
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We seek an energetic and motivated Scientist II to join our Immuno-Oncology team, which is aiming to redirect the immune system towards tumors by engineering hematopoietic cells in vivo. Experience with typical in vitro methodologies to measure immune cell function, including flow cytometry, ELISA, real-time microscopy, etc.
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Experience with standard immunological assays such as flow cytometry / FACS, magnetic cell sorting, ELISA/Luminex, immunofluorescence staining and confocal microscopy, cell proliferation assays, cytotoxicity assays.
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Extensive experience in preclinical in vivo evaluation of T cell therapies with coupled expertise in ex vivo T-cell pharmacokinetic and pharmacodynamic readouts such as T-cell persistence, immunophenotype, biodistribution (by flow cytometry and IHC), cytokine releaseFirsthand expertise in ex vivo immunoprofiling using ELISA and flow cytometry, with high favourability toward experience with adoptive T cells.
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Effectively execute experiments evaluating both in vitro and ex vivo immune readouts, using ELISA, flow cytometry, and other related techniques. Extensive expertise in ELISA (or similar assays), flow cytometry and familiarity with basic molecular biology techniques.
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Design and execute cell-based studies to support identification, validation, and differentiation of small molecule protein degraders, implementing a wide variety of laboratory techniques including mammalian cell culture, Western blotting, CRISPR, RNAi, phenotypic assays, flow cytometry, etc.
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Perform basic cell culture and in vitro assays and analyze by multi-parameter flow cytometry. Under general supervision, will breed and maintain common use mouse strains and use molecular (PCR) and cellular (flow cytometry) techniques to genotype transgenic and knockout mice.
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Experience with techniques such as multi-parameter flow cytometry, cell-based immunological assays using primary cells, ELISA/MSD, HTRF, TR-FRET, and other relevant assays to monitor compound activity/MoA.
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vivo cytometry jobs in Boston, MA
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