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Research Associate, Protein Science, protein expression, purification, microbial cell culturing, molecular cloning, biochemical synthesis, FPLC, SDS-PAGE, ELISA, colorimetric assays, Microsoft Office.
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The successful applicant will assist senior lab staff with studies of murine-based projects that involve techniques such as exosome production and isolation, western blot, RNA/DNA extraction, PCR, flow cytometry, IHC, ultracentrifugation, cell culture, mouse surgery and husbandry.
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Perform research on projects working with nucleic acid purification, qPCR, molecular cloning, next-generation sequencing, Western Blot, ELISA, fluorescence microscopy, and flow cytometry. + Experience with library preparation for next-generation sequencing, CRISPR screens and RNA Seq is preferred.
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The primary responsibilities of the RAs will include isolation and culture of primary human immune cells, functional analysis of engineered cells via high throughput flow cytometry, assistance with genomic techniques such as single-cell RNA sequencing and CRISPR gene editing, among others.
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Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR. Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR.
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Hands-on expertise in tissue culture and cell-based assays (including general tissue culture, cell banking, cell proliferation, electroporation, transfection, transduction, flow cytometry, AAV, and ELISA.
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Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing. Experience with biochemical analysis techniques, including Western Blot, ELISA, Enzyme activity assays, Immunostaining.
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Analytical methods may include, but not limited to: SE-HPLC, CEX-HPLC, HILIC-HPLC, reduced and nonreduced CE-SDS, cIEF, concentration by A280, and ELISA assays. Coordinate with other functional groups to support upstream and downstream process development studies and have some understanding and experience with determining critical quality attributes of protein products.
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Proficient in protein-based assay development including ELISA, enzymatic activity assay, AlphaLisa, etc. Proficient in protein-based assay development including ELISA, enzymatic activity assay, AlphaLisa, etc.
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3+ years of experience in NGS and with extensive hands-on experience in the full workflow from DNA/RNA extraction to sequencing-ready library. Using your expertise in cancer genomics and techniques like DNA/RNA extraction, library preparation, and sequencing, you will help push our therapeutic discovery and development forward.
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Tissue and cell culture, RNA/DNA purification, cDNA preparation, quantitative PCR, Western blotting, etc. Tissue and cell culture, RNA/DNA purification, cDNA preparation, quantitative PCR, Western blotting, etc.
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Experience analyzing in vivo samples (ELISA, MSD, etc.) In this position, you will assist with the design and execution of in vivo pharmacology experiments in rodents. Experience analyzing in vivo samples (ELISA, MSD, etc.
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Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets. Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets.
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Experience with multi-color flow cytometry, T cell functional assays, ELISA assays, culture of mammalian cell lines and primary immune cells is required. Experience with multi-color flow cytometry, T cell functional assays, ELISA assays, culture of mammalian cell lines and primary immune cells is required.
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Molecular biology and biochemistry: RNA and DNA extractions, cloning, mutagenesis, quantitative PCR, protein immunoprecipitations, Western blotting, and proteomic sample preparation. Stem cell culture: CRISPR editing, stem cell culture, directed differentiation, and fluorescence microscopy of human induced pluripotent stem cells (hiPSCs.
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