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Perform research on projects working with nucleic acid purification, qPCR, molecular cloning, next-generation sequencing, Western Blot, ELISA, fluorescence microscopy, and flow cytometry. + Experience with library preparation for next-generation sequencing, CRISPR screens and RNA Seq is preferred.
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Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR. Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR.
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Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing. Experience with biochemical analysis techniques, including Western Blot, ELISA, Enzyme activity assays, Immunostaining.
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The successful applicant will assist senior lab staff with studies of murine-based projects that involve techniques such as exosome production and isolation, western blot, RNA/DNA extraction, PCR, flow cytometry, IHC, ultracentrifugation, cell culture, mouse surgery and husbandry.
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The primary responsibilities of the RAs will include isolation and culture of primary human immune cells, functional analysis of engineered cells via high throughput flow cytometry, assistance with genomic techniques such as single-cell RNA sequencing and CRISPR gene editing, among others.
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3+ years of experience in NGS and with extensive hands-on experience in the full workflow from DNA/RNA extraction to sequencing-ready library. Using your expertise in cancer genomics and techniques like DNA/RNA extraction, library preparation, and sequencing, you will help push our therapeutic discovery and development forward.
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Experience analyzing in vivo samples (ELISA, MSD, etc.) In this position, you will assist with the design and execution of in vivo pharmacology experiments in rodents. Experience analyzing in vivo samples (ELISA, MSD, etc.
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Tissue and cell culture, RNA/DNA purification, cDNA preparation, quantitative PCR, Western blotting, etc. Tissue and cell culture, RNA/DNA purification, cDNA preparation, quantitative PCR, Western blotting, etc.
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Proficient in protein-based assay development including ELISA, enzymatic activity assay, AlphaLisa, etc. Proficient in protein-based assay development including ELISA, enzymatic activity assay, AlphaLisa, etc.
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Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets. Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets.
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Experience with multi-color flow cytometry, T cell functional assays, ELISA assays, culture of mammalian cell lines and primary immune cells is required. Experience with multi-color flow cytometry, T cell functional assays, ELISA assays, culture of mammalian cell lines and primary immune cells is required.
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Molecular biology and biochemistry: RNA and DNA extractions, cloning, mutagenesis, quantitative PCR, protein immunoprecipitations, Western blotting, and proteomic sample preparation. Stem cell culture: CRISPR editing, stem cell culture, directed differentiation, and fluorescence microscopy of human induced pluripotent stem cells (hiPSCs.
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Must have experience with DNA/RNA handling and purification. Existing collaborations within CZ Biohub: Technology Platforms (Bioengineering, Computational Microscopy, Data Science, Genomic Sequencing, Mass Spectrometry/Proteomics), and Research Group Leaders.
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The candidate will join the Process Science analytical team to study RNA folding computationally and experimentally for its CRISPR by Design platform. In-silico RNA structural modeling and alternative RNA structure ensemble deconvolution.
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Proficiency in a broad range of analytical techniques used in cell and gene therapies (qPCR/dPCR, Flow Cytometry, ELISA, HPLC) The Scientist 2/Senior Scientist in Analytical Development will assume a pivotal role within GeneFab’s Technical Development department, executing against and supporting oversight of the analytical functions (method development, qualification, routine testing, and new technology evaluation) that are integral to the successful development and manufacturing of cell and gene therapy products for new or existing clients.
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