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You will perform various techniques involving tissue culture of mammalian cells (human and murine) with sterile aseptic techniques and will perform DNA/RNA/Protein isolation/extraction/quantification from assorted biological material and tissue.
$28.04 an hourFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Perform techniques involving DNA, RNA, and protein extraction from human biological materials of different types; generate DNA plasmids for cell culture studies. JOB QUALIFICATIONSQualificationsPrevious lab experience, knowledge of standard laboratory equipment and supplies, knowledge of tissue culture and sterile technique, knowledge of neural stem cell culture preferred, knowledge of chemistry to prepare common lab solutions, knowledge of laboratory safety procedures for handling chemical and biohazardous waste, knowledge of basic molecular biology techniques, general knowledge of University procedures.
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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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You will also perform DNA/RNA/protein isolation/extraction/quantification from assorted biological material and tissue, as well as cell culture work, DNA/RNA/protein assays, research data procurement, and data analysis.
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Under immediate supervision work rotations are performed in all areas of a full service genetic lab for a specialized Molecular GeneticS laboratory including: DNA and RNA extraction, Polymerase Chain Reaction (PCR), Reverse Transcriptase PCR, Southern Blotting, agarose gel electrophoresis, restriction digest, Capillary Electrophoresis, Sanger Sequencing, Probe Hybridization, Bead Capture technology, Microarray technology and more.
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3 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting. 4 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting.
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In vivo research: rodents handling and basic manipulations and blood processing to serum and plasmaIn vitro techniques: cell line expantion and cell culture under different treatment conditionsMolecular biology: RNA isolation and purification, qPCRBiomarkers: ELISAs, WESTERN, protein extraction from cell cultures and animal tissues, Luminex-based multiplex platform (as a plus.
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