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Preferred with at least 1 year molecular lab experience including DNA/RNA extraction, library preparation and NGS analysis. Perform various molecular tasks including nucleic acid extraction, PCR, library preparation, sequencing workflow executions and analysis according to established laboratory procedures.
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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Skills in tissue dissections and molecular biology techniques, including working with DNA, protein (immunoprecipitation and Western blotting), RNA extraction, qPCR and qRT-PCR analysis, and standard biochemical assays.
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing. Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing.
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Execute and analyze molecular assays designed to determine gene editing outcomes (nucleic acid extraction, PCR, Sanger-sequencing, NGS library preparation, Amplicon-seq, Flow cytometry). Extensive hands-on experience with Molecular Biology (as PCR, qRT-PCR, ddPCR, DNA/RNA and protein preparation), Cloning, mammalian cell culture and transfection is required.
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3 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting. 4 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting.
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Required technical skills include: RNA and DNA extraction, tissue culture, and flow cytometry techniques. Required technical skills include: RNA and DNA extraction, tissue culture, and flow cytometry techniques.
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Proficient in molecular techniques e.g. cloning, DNA/RNA extraction. Perform basic cloning, plasmid, RNA, and gDNA isolation. Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
$75,000 - $98,000 a yearExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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60%) Molecular/cell biology techniques including aseptic cell culture, treatment of cancer cells with anti-cancer drugs, cell viability assay, extraction of RNA/protein, running qRT-PCR and/or Western immunoblotting as well as flow cytometry-based analysis of cells and proteins.
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Hands-on experience with cell-based assays and general molecular biology methods including, but not limited to, cell transfection, IVT, RNA extraction, qPCR, ddPCR, FACS, TapeStation, or Fragment Analyzer.
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Perform experiments involving PCR, Quantitative PCR, Western Blots, PCR Arrays, RNA and DNA extraction, protein expression, and purification. The incumbent will perform experiments involving PCR, Real-time PCR, Northern and Western Blots, PCR Arrays, RNA and DNA extraction, protein expression, and purification.
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Biomarkers: ELISAs, WESTERN, protein extraction from cell cultures and animal tissues, Luminex-based multiplex platform (as a plus) Molecular biology: RNA isolation and purification, qPCR.
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Experience handling tissue, including dissociation, isolation, single cell/nuclei suspension preparation, DNA and RNA extraction and quantification; NYGC scientists and staff are furthering new approaches to diagnosing and treating neurological diseases and cancer through their unique capabilities in whole genome sequencing, RNA sequencing, state-of-the art analytics, and the development of genomic tools.
$71,000 - $88,000 a yearFull-timeExpandUpdated Today - UpvoteDownvoteShare Job
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Planning and executing experiments using genetics, molecular biology (PCR, restriction digest, gel electrophoresis, RNA extraction, qRT-PCR, CRISPR-based editing, DNA microinjections), basic biochemistry techniques (immunoprecipitations, Western blotting), behavioral experiments, fluorescence microscopy, and quantitative image analysis.
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Experiments include, but not limited to qRT-PCR, western blotting, mammalian cell culture and cell line maintenance, DNA/RNA extraction, electrophoresis, ELISA, proliferation, and migration assays, Ca2 imaging, isolated and pressurized arterioles from mouse brain, patch-clamp electrophysiology on isolated cells, in vivo two-photon microscopy.
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