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The successful candidate must have previous experience and be proficient in mouse husbandry, PCR-based genotyping, cell culture, flow cytometry, as well as DNA, RNA, and protein isolation and detection techniques.
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Additionally, the specialist will assist with next-generation sequencing, cell culture, and the development and implementation of high-quality polychromatic flow cytometry and T cell-based assays.
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How You Will Achieve ItBe a key member of the Cell Line Development team that will support the execution of both technology development and pipeline projects, including vector construction, maintenance of host cells, transfection, clone selection and cell line characterization, cell bank thawing/freezingPerform molecular cloning, DNA prep and RNA isolation.
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Required technical skills include: RNA and DNA extraction, tissue culture, and flow cytometry techniques. Areas of expertise include genomics, epigenetics, drug resistance, stem cell biology, drug discovery, diagnostics and biomarker development, and pharmacology.
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The applicant will train students and junior members of the laboratory in equipment operation and laboratory techniques, including flow cytometry, mass cytometry, PCR, ELISA, biochemistry techniques and cell culture among others.
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The Associate Research Specialist will perform molecular biology techniques (cloning, plasmid generation, DNA and RNA isolations), protein work including Western blots, generate lentivirus, culture neurosphere cell lines, and perform functional genomics experiments in these cell lines.
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Informed interpretation of experimental data and planning for subsequent experiments Bachelor's (University) degree in biology, chemistry, biochemistry or microbiology and research laboratory with documented research laboratory experience (1 academic year outside of coursework) in cell culture sterile technique, molecular cloning, genetics, and proteomics.
$50,620 a yearExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Assisting with a variety of experimental methods including but not limited to: DNA/RNA isolations, PCR, qPCR, cell culture, and isolation of protein. Processing a variety of mouse/human samples-blood, tissue, and cell lines.
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Procedures include but are not limited to: isolation and analysis of DNA, RNA, and protein from cultured cells and animal tissues; mass spectrometry sample preparation and data analysis; and primary cell culture and mouse handling and experimentation.
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Research techniques involve gel electrophoresis, preparation of DNA and RNA, plasmid cloning, preparation of microbial cells, preparation of recombinant media and appropriate growth conditions, tissue culture maintenance and transfection as well as imaging using state of the art microscopy techniques and perform image analysis and data management.
$21.39 - $23.06 an hourFull-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Training in developmental biology, cell culture, microscopy, and fish physiology. Knowledge and experience in molecular biology techniques including genotyping, PCR, DNA/RNA isolation, primer design, qPCR, and in vitro RNA synthesis.
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Work will involve, among other techniques, immunohistochemistry, imaging with confocal microscope, quantitative real-time PCR, Western blotting, DNA/RNA extraction, subcloning of plasmids, iPSC culture, iPSC transfection etc.
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
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Establishing in vitro assays to evaluate efficacy, dose response relationships, and characterizing molecules for potential therapeutic effects via techniques including cell culture, qPCR, transfection, transduction, cholesterol measurement, RNA expression analysis, and general molecular biology.
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