Portland VA Research Foundation

Analysis of viral growth by microscopy and enzyme assays.. Viral DNA, RNA and protein extraction, purification, and analysis, including cloning, amplification and DNA sequencing.. Sample preparation for DNA sequencing.. Participate in laboratory and biosafety training and maintain safe laboratory practices.. Informed interpretation of experimental data and planning for subsequent experiments Bachelor's (University) degree in biology, chemistry, biochemistry or microbiology and research laboratory with documented research laboratory experience (1 academic year outside of coursework) in cell culture sterile technique, molecular cloning, genetics, and proteomics.

research assistant ii

Job Title: Research Assistant II FTE: 1.0 Salary Range: $50,620/annually Position Summary Assist in research on genetic alterations related to antiviral drug resistance in human cytomegalovirus. Characterize the viral mutations responsible for a drug-resistant phenotype and associated changes in growth and biological function of the virus. ESSENTIAL DUTIES AND RESPONSIBILITIES Perform viral and cell culture using aseptic techniques. Analysis of viral growth by microscopy and enzyme assays. Viral DNA, RNA and protein extraction, purification, and analysis, including cloning, amplification and DNA sequencing. Sample preparation for DNA sequencing. Construction of recombinant viral strains containing designated mutations. Assays for viral drug susceptibility, growth properties, antigen and gene expression. Participate in laboratory and biosafety training and maintain safe laboratory practices. Acquisition and preparation of starting materials and reagents for research. Accurate and timely data entry and maintenance of laboratory records and electronic databases. QUALIFICATIONS Required: Quality control activities including continuous review and modification of experimental techniques, laboratory records, specimen archives, and databases. Timely ordering, acquisition and preparation of starting materials for laboratory research, including reagents, cultures, glassware and plasticware. Maintain records of supplies and reagents ordered and received. Cleaning and sterilization of materials using on-premises equipment. Operation and maintenance of specialized equipment including microscopes, centrifuges, spectrophotometers, balances, digital cameras, and computers. Informed interpretation of experimental data and planning for subsequent experiments Bachelor's (University) degree in biology, chemistry, biochemistry or microbiology and research laboratory with documented research laboratory experience (1 academic year outside of coursework) in cell culture sterile technique, molecular cloning, genetics, and proteomics. Ability to operate standard molecular biology equipment. LANGUAGE SKILLS Ability to read and interpret English language technical documents without difficulty, such as safety regulations, operating and maintenance instructions, research papers, and procedure manuals. Ability to write routine reports and make accurate entries in laboratory notebooks and databases. Efficient English verbal communication and understanding of experimental plans, procedures, observations, and results. MATHEMATICAL SKILLS Ability to add, subtract, multiply, and divide in all units of measure, using whole numbers, common fractions, and decimals. Ability to compute serial dilutions, mean, median, standard deviation, rate, ratio, and percent, and to draw and interpret line charts. Understanding of the meaning of units of concentration such as nanomolar and micromolar, their essential distinction and interconversion. COMPUTER SKILLS Job absolutely requires specialized computer skills which will be in constant use. Must be adept at using various applications including database, spreadsheet, word processing, and e-mail. Adept at using Microsoft office suite including Excel and Access. Awareness of network-specific requirements for use of multi-user databases. REASONING ABILITY Ability to apply common sense in situations involving safety and integrity of persons, environment, materials and data, consistent with the required level of education and research experience. Sufficient understanding to carry out instructions competently, when furnished in written, oral, or diagram form at a level of detail expected for those with the required educational and technical background. Ability to deal with problems involving several concrete variables in standardized situations. Apply online at and include a CV and cover letter explaining your interest in the position . Cover letter should include a description of recent work history as it relates to the required qualifications for this position. Portland VA Research Foundation is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to status as a protected veteran or a qualified individual with a disability, or other protected status, such as race, religion, color, national origin, sex, age. Job Posted by ApplicantPro

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