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Skills: Small animal surgery, mouse colony management, genotyping, DNA and RNA extraction, PCR, cloning, tissue cryosection, immunohistochemistry, and lab organization management. Skills: Small animal surgery, mouse colony management, genotyping, DNA and RNA extraction, PCR, cloning, tissue cryosection, and immunohistochemistry.
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Under the general supervision of the investigator, performs complex laboratory experiments in the field of cell biology including: cell culture, ELISA, DNA/RNA isolation, PCR, general biochemistry, immunohistochemistry, flow cytometry, and microscopy.
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Laboratory bench protocols including RNA/protein extraction, western blotting, PCR, histology, ELISAs, microscopy, or other biochemical assays. This will include but is not limited to Western blots, RT-PCR, immunohistochemistry, ELISAs, cell culture, etc.
$34,320 - $52,988Full-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Designs and executes scientific experiments which frequently utilize methods such as protein detection and quantification, enzymatic and other cell-based test methods, immunohistochemistry, microscopic image analyses, and biomaterials testing.
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Conducts male reproductive tissue dissection and processes histology and immunohistochemistry. Performs human and mouse DNA and RNA isolation for PCR and high-throughput sequencing techniques.
$28,710 - $38,830 a yearFull-timeExpandApply NowActive JobUpdated 1 month ago - UpvoteDownvoteShare Job
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Experience performing qPCR, qRT-PCR, DNA sequencing, ChIP-seq, mRNA isolation, northern blots, cDNA synthesis, immunoblots, co-immunoprecipitation, immunohistochemistry, and in situ hybridizations, are highly desired.
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General duties will include breeding, weaning and genotyping genetically altered mice, assisting in collection, processing and cataloging tissue samples from mice, culture of primary cells, RNA and protein extraction, performing various biologic analyses (gel electrophoresis, Western blotting, ELISA, immunohistochemistry and immunocytochemistry, qRT-PCR, etc.
$31,561 - $58,393 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Key Responsibilities: 70% - Mammalian cell culture; RNA and DNA isolation; qRT-PCR, shRNA techniques; protein isolation and immunoprecipitation-western blotting; immunohistochemistry; in situ hybridization; fresh frozen and formalin-fixed paraffin-embedded tissue preparation and sectioning; and other histological/molecular/cellular biology techniques required for the research projects.
$42,088 - $53,536Full-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Cell culture maintenance under faculty supervision: Generation, propagation and maintenance of sterile cell cultures and intestinal organoids, performing basic in vitro experiments, including but not limited to harvesting, splitting, and freezing cells, drug delivery to cultures, protein, and RNA extractions.
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Responsibilities also include supporting the lab’s research activities by performing a variety of procedures including but not limited to: literature reviews, specialized cell and tissue isolations and cultures; ELISAs; protein and RNA extraction from cells and tissues; flow cytometry; immunohistochemistry and immunofluorescence; Western blotting; RT-qPCR; mouse colony management; animal surgeries and tissue collections.
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Trained to perform genetic, biochemical, molecular, and cellular techniques in experimental disease model systems including but not limited to: DNA/RNA/protein extraction and quantification, SDS-PAGE/immunoblot, PCR, real-time qPCR, immunofluorescent and immunohistochemistry, biochemical assays, cell culture, rodent colony management, transgenic rodent models, rodent exposures, and mammalian tissue collection and processing.
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Extracts DNA, RNA, protein from fresh/frozen and formalin fixed paraffin embedded samples. Experience with DNA array profiling or immunofluorescence/immunohistochemistry techniques. Experience with microscopy such as confocal microscopy or Raman spectroscopy.
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This position will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Tissue staining, immunohistochemistry, western blotting, ELISAs, RNA work. These tests are performed in the areas of chemistry, hematology or blood, immunology, microbiology, molecular diagnostics, toxicology and transfusion medicine.
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Experience with immunohistochemistry, cell culture (organoid culture specifically would be useful), and/or flow cytometry. Depending on interest, additional responsibilities will likely include collection and processing of biological specimens, isolation and culture of primary human vascular and neural cells, as well as functional analysis via flow cytometry, imaging, and RNA sequencing.
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