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Experience with flow cytometry, mass cytometry (CyTOF), and/or immunohistochemistry. Performing flow cytometry and immunohistochemistry experiments. Performing a range of molecular biology techniques, including molecular cloning, DNA and RNA isolation, and protein expression analysis.
ExpandApply NowActive JobUpdated 5 days ago - UpvoteDownvoteShare Job
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Molecular biology and biochemistry: RNA and DNA extractions, cloning, mutagenesis, quantitative PCR, protein immunoprecipitations, Western blotting, and proteomic sample preparation. Stem cell culture: CRISPR editing, stem cell culture, directed differentiation, and fluorescence microscopy of human induced pluripotent stem cells (hiPSCs.
Full-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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3+ years of experience in NGS and with extensive hands-on experience in the full workflow from DNA/RNA extraction to sequencing-ready library. Using your expertise in cancer genomics and techniques like DNA/RNA extraction, library preparation, and sequencing, you will help push our therapeutic discovery and development forward.
ExpandApply NowActive JobUpdated 7 days ago - UpvoteDownvoteShare Job
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You will be involved in developing and deploying high-throughput RNA production and purification platform assist in RNA production to ensure smooth operation. You will get to work on the cutting edge of biotech research in this exciting and exclusive role, working on the development and refinement of RNA constructs for a variety of therapeutic products.
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Strong background in assay development using molecular genomic technologies (both RNA and DNA), including experience in next-generation sequencing, qPCR, microarray or other high-throughput platforms.
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ResponsibilitiesExtract protein, DNA and/or RNA from necropsied animal tissueRun ddPCR, qPCR, ELISA, Western Blot etc analysis on tissue extractsCell culture, Neutralizing antibody assays and total antibody assaysSample analysis assay optimizationSOP writingData recording and analysisQC analysis This position directly impacts preclinical outcomes including proof of concept, ED50, MED, clinical hold response and IND enabling studies.
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Define and support the implementation of CQdriven analytical strategies, oversee analytical method development, qualification, validation and Tech Transfer of lot release, stability, in-process and characterization methods for a diverse range of RNA platforms both in-house and at CMOs/CROs/CTLs.
ExpandApply NowActive JobUpdated 3 days ago - UpvoteDownvoteShare Job
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Demonstrated experience with routine molecular biology techniques (DNA/RNA/protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence) Based on our innovative approach to developing genetic medicines, as well as our industry-leading internal manufacturing capability and expertise, we have become the Astellas Center of Excellence for their newly created Genetic Regulation Focus Area. We are currently exploring three gene therapy modalities: gene replacement, exon skipping gene therapy, and vectorized RNA knockdown, with plans to expand our focus and geographic reach under Astellas.
ExpandApply NowActive JobUpdated 8 days ago - UpvoteDownvoteShare Job
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In vitro experience with cultured cell lines, siRNA transfection techniques, CRISPR, western blotting, quantitative PCR techniques, RNA/DNA isolation, scRNAseq, laboratory automation, high-content imaging technology, and fluorescence microscopy.
Full-timeExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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Experience with immunohistochemistry, cell culture (organoid culture specifically would be useful), and/or flow cytometry. Depending on interest, additional responsibilities will likely include collection and processing of biological specimens, isolation and culture of primary human vascular and neural cells, as well as functional analysis via flow cytometry, imaging, and RNA sequencing.
ExpandApply NowActive JobUpdated 14 days ago - UpvoteDownvoteShare Job
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing. Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing.
ExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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The UCSF immunohistochemistry lab offers a wide array of biomarkers and continually adds emerging ones. The UCSF Clinical Cancer Genomics Lab runs targeted gene sequencing, microsatellite instability testing, fluorescence in situ hybridization (FISH), and a 500+ gene next-generation sequencing panel for advanced diagnostic and prognostic molecular testing in patients with cancer.
$250,000 - $360,000ExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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TCR repertoire analysis from DNA and RNA sequencing data. 4+ years of experience in computational biology for immuno-oncology applications (academic experience during PhD is counted). We are currently looking for a computational cancer biologist in the field of immuno-oncology to join our interdisciplinary team.
ExpandApply NowActive JobUpdated 10 days ago - UpvoteDownvoteShare Job
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Experience with assay development in RNA therapeutics such as high-throughput RT-qPCR RNFISH and characterization of in-cell RNA binding. Broad experience and expertise in molecular reagent generation and cell line engineering using gene editing approaches CRISPR as well as development and implementation of novel cellular target engagement activity and PPI assays e.g. immunoassays second messenger generation ion flux reporter protein stability PPI splitNanoLuc split-gal gene activation cellular imaging.
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Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets. Experience with analyzing single-cell RNA sequencing, proteomics or pooled CRISPR screening datasets.
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immunohistochemistry rna jobs in San Francisco, CA
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