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Prior training in immunohistochemistry and sterile cell culture. culture, PCR, primer design, and RNA extraction. Prior training in immunohistochemistry and sterile cell culture. culture, PCR, primer design, and RNA extraction.
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Perform immunological assays that include ELISA, ELISPOT, Luminex assays, flow cytometry, cell-based neutralization assays, immunohistochemistry. Experience handling large data sets, statistics, software tools (Word, Excel, PowerPoint)Expertise in molecular biology techniques including plasmid DNA cloning, RNA synthesis, sequencing, microarray, single cell genomics, RNASeq.
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Expertise in molecular biology techniques including plasmid DNA cloning, RNA synthesis, sequencing, microarray, single cell genomics, RNASeq. Produce RNA constructs through in vitro transcription, then test antigen expression through mass spectrometry and Western blot.
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Trainees will perform basic and translational research involving molecular biology approaches (such as CRISPR/Cas technology, Western blotting, ELISA, and immunohistochemistry), in vitro assays (such as drug screening, flow cytometry, and development of resistant cell lines), and in vivo experiments using xenograft, syngeneic and genetically engineered mouse models of KRAS-mutant lung cancer.
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Experience independently working with endothelial, myeloid and tumor cell culture, DNA and RNA isolation from culture cells and from tissues, immunoblotting and quantitative RT-PCR for genes and proteins that regulate myeloid cell adhesion, migration and gene and protein expression in vitro and in vivo.
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Experimental Support: Assisting in experiments as required, potentially involving surgical procedures, histology, immunohistochemistry, in situ hybridization, microscopy, and computer-assisted analysis; RNA sequencing and tissue culture.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linked immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linker immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Work will involve, among other techniques, immunohistochemistry, imaging with confocal microscope, quantitative real-time PCR, Western blotting, DNA/RNA extraction, subcloning of plasmids, iPSC culture, iPSC transfection etc.
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Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to mammalian cell culture, DNA and RNA isolation, PCR, immunohistochemistry, DNA assembly, bacterial transformation.
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Expertise in molecular and cellular biology techniques, such as ddPCR, virus production and transductions, confocal microscopy, immunohistochemistry, flow cytometry. Proficiency in RNA and DNA purification, qPCR, cloning and mammalian cell culture.
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Extraction of RNA, DNA, and protein with analysis using RNAseq, PCR, genotyping, western blot, and immunohistochemistry. Extraction of RNA, DNA, and protein with analysis using RNAseq, PCR, genotyping, western blot, and immunohistochemistry.
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Utilize common molecular and cell biology skills including Western Blot, ELISA, RNA quantification methods (i.e. Q-PCR), and immunohistochemistry/immunofluorescence to support preclinical development of compounds that will be entering clinical development.
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As part of our mission, TRACTION is focused on partnering with our internal drug discovery engine (IACS, ORBIT, Biologics Development and Cell Therapies) and a growing list of external Partners (Boehringer Ingelheim, Taiho, Blueprint Medicines, Schrödinger and Obsidian) to discover and develop the next generation of small-molecule targeted therapies, antibody therapeutics and cell therapies, all driven by the needs we see in our patients.
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Our work is based on our single cell RNA sequencing and spatial profiling efforts that revealed several immunologically relevant and spatially-organized multicellular interaction networks, 'immune hubs', in tumor tissues from patients with colorectal cancer (Pelka et al. Cell 2021, Tian et al. Nat Med 2023, Chen et al. BioRxiv 2023.
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