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Perform research on projects working with nucleic acid purification, qPCR, molecular cloning, next-generation sequencing, Western Blot, ELISA, fluorescence microscopy, and flow cytometry. + Experience with library preparation for next-generation sequencing, CRISPR screens and RNA Seq is preferred.
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Hands-on experience with molecular cloning, plasmid preparation, RNA isolation and other molecular biology techniques. Experience with bacterial cultures and ELISA assays. Hands-on experience with molecular cloning, plasmid preparation, RNA isolation and other molecular biology techniques.
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Proficiency in mammalian cell culture techniques, flow cytometry, qPCR, DNA and RNA extraction, transfection, ELISA, and western blotting. The current Flagship ecosystem comprises 41 transformative companies, including Axcella Health (NASDAQ: AXLA), Denali Therapeutics (NASDAQ: DNLI), Evelo Biosciences (NASDAQ: EVLO), Foghorn Therapeutics (NASDAQ: FHTX), Indigo Ag, Kaleido Biosciences (NASDAQ: KLDO), Moderna (NASDAQ: MRNA), Rubius Therapeutics (NASDAQ: RUBY), Sana Biotechnology, Seres Therapeutics (NASDAQ: MCRB), and Sigilon Therapeutics (NASDAQ: SGTX.
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RNA isolation and analysis by qRT-PCR, molecular biology (PCR, genotyping, cloning plasmid constructs, DNA isolation and sequencing analysis, CRISPR). With respect to laboratory technical skills, the individual must be proficient in cell/tissue culture, flow cytometry/sorting (FACS), protein isolation and analysis (western blot, ELISA, and.
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Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR. Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR.
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In our Brighton/Boston labs, RNA biology meets today's cutting-edge genomics, computational biology, and deep-sequencing technologies to create a new class of medicines that address the underlying causes of disease.
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Proficiency in standard tumor immunology techniques including DNA and RNA analysis, T cell cloning, TCR analysis, Elispot, Elisa, and T cell CAR therapy. Employ state-of-the-art molecular biology techniques to analyze gene expression patterns in vivo, including qRT-PCR, RNA sequencing, and single-cell RNA sequencing, to identify molecular signatures associated with therapeutic response.
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Broad technical expertise/knowledge including with flow cytometry, ELISA, immunohistochemistry, qRT-PCR, NGS and RNA Seq. Broad technical expertise/knowledge including with flow cytometry, ELISA, immunohistochemistry, qRT-PCR, NGS and RNA Seq.
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Experiments include, but not limited to qRT-PCR, western blotting, mammalian cell culture and cell line maintenance, DNA/RNA extraction, electrophoresis, ELISA, proliferation, and migration assays, Ca2+ imaging, isolated and pressurized arterioles from mouse brain, patch-clamp electrophysiology on isolated cells, in vivo two-photon microscopy.
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Proper labeling, handling, and storage of serum, plasma, DNA, and RNA samples. Running ELISA assays on human blood and urine samples. This person will focus on the implementation and analysis of individual research projects that will include measuring biomarkers using ELISA assays and proteomic platforms.
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Biochemistry and protein chemistry experience (flow cytometry, FACS, ELISA, western blotting, SDS-PAGE, etc.) Strong molecular biology background with a proficiency in DNA/RNA isolation and purification, cloning, sub-cloning, PCR, RT-qPCR, genotyping, expression construct design, transfection, CRISPR gene editing, and RNA profiling.
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Will perform routine cellular and molecular assays (e.g., transformation, miniprep, maxiprep, RNA, DNA and protein preparation, cDNA synthesis, qRT-PCR, transfection, packaging of viral vectors, ELISA, Western Blotting, etc.
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Avidity Biosciences, Inc.'s mission is to profoundly improve people's lives by delivering a new class of RNA therapeutics - Antibody Oligonucleotide Conjugates (AOCs™). Avidity is revolutionizing the field of RNA with its proprietary AOCs, which are designed to combine the specificity of monoclonal antibodies with the precision of oligonucleotide therapies to address targets and diseases previously unreachable with existing RNA therapies.
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Primary responsibilities may include buffer preparation, drug formulation, endotoxin testing, animal tissue processing, DNA/RNA/protein isolation, spectrophotometry, chromatography, immunochemistry, microscopy, image analysis, enzyme activity assays, protein/oligo/small molecule interaction techniques (ELISA, SPR, BLI, MST, FRET, etc), various molecular biology techniques (PCR, qPCR, ddPCR, dPCR, SDS-PAGE, etc.
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Arcturus' commitment to the development of novel RNA therapeutics has led to collaborations with Janssen Pharmaceuticals, Inc., part of the Janssen Pharmaceutical Companies of Johnson & Johnson, Ultragenyx Pharmaceutical, Inc., Takeda Pharmaceutical Company Limited, CureVac AG, Synthetic Genomics Inc., Duke-NUS, and the Cystic Fibrosis Foundation.
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