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Experience in working with in vitro viral transduction assays, FACS and various cell/molecular techniques (transfection, RNA/protein analysis, immunostaining) are preferred. Running and troubleshooting assays, including ELISA, FACS, Western blotting, qPCR, and fluorescence microscopy imaging.
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Proficiency in molecular biology techniques such as DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, and Next Generation Sequencing. Proficiency in molecular biology techniques such as DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, and Next Generation Sequencing.
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Molecular biology skills (extraction of RNA, PCR cloning and nucleotide point mutagenesis, etc.) Cell culture studies (analysis of cell cycle progression, proliferation, differentiation or death using assays such as Coulter counting, MTT, FACS, TUNEL, ELISA, etc.
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In addition, review and approve CTL assay development and validation reports, relevant sections of clinical documents pertaining to AAV and RNA analytical testing and documentation for submission to regulatory agencies.
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Conduct assays and experiments using flow cytometry, PCR, cell-based assays/bioassay development, and ELISA techniques. Demonstrated experience with standard molecular biology techniques (DNA/RNA/protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence.
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Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing. Experience with biochemical analysis techniques, including Western Blot, ELISA, Enzyme activity assays, Immunostaining.
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
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Knowledge of molecular biology techniques (DNA/RNA isolation, PCR, Elisa, and sequencing). Knowledge of molecular biology techniques (DNA/RNA isolation, PCR, Elisa, and sequencing). This position will be in support of research conducted in the laboratories of Dr. Morgane Rolland at the U.S. Military HIV Research Program in the Viral Genomic Section and Systems Serology Core: Dr. Rolland's Lab. We're looking for someone who is organized and detail-oriented who enjoys working with a supportive and inclusive team.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Analysis may include immunological procedures including Enzyme Linked immunoassay (ELISA), Western blot, Flow Cytometry and RNA/DNA sequencing assays. Operate and analyze data collected on Western Blot equipment such as Protein Simple WES, DNA fragmentation using Covaris systems, Real-Time PCR Detection System such as BioRad CFX, and quantification and QC of RNA and DNA using NanoDrop 8000, Qubit 3.0 Fluorometer, and Agilent 2200 and 4200 TapeStation systems.
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Various assays performed on purified populations including one or more of the following: Immunoflorescent staining ELISPOT ELISA RNA isolation/RT-PCR Prepares stock solutions and reagents.
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Experience independently working with endothelial, myeloid and tumor cell culture, DNA and RNA isolation from culture cells and from tissues, immunoblotting and quantitative RT-PCR for genes and proteins that regulate myeloid cell adhesion, migration and gene and protein expression in vitro and in vivo.
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Examples of assays include ELISA, Western blot, viral plaque assay, flow cytometry, and PCR. Performs research with molecular/cell biological and histological techniques including PCR, DNA and RNA extraction, immunostaining, Western-blotting, cell culture, and preparing cryosections.
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Experience in basic molecular biology (i.e. RNA,/DNA/plasmid extraction, qPCR, ddPCR, etc.) Experience with ELISA, qPCR, ddPCR, SDS-PAGE, and other non-cell based methods. Experience in basic molecular biology (i.e. RNA,/DNA/plasmid extraction, qPCR, ddPCR, etc.
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Hands-on experience with standard molecular and cell biology techniques including qPCR, ELISA, flow cytometry, and cell culture. Knowledge of current advances in DNA extraction, RNA and DNA sequencing (desired but not required.
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