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Flow cytometry, ELISA, cytokine assays, cell culture, DNA/RNA isolation, protein isolation/purification and western blot, animal handling and basic surgical procedures in mice. DNA, RNA, Protein isolation, purification and expression.
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Expertise in molecular biology (PCR, RT-PCR, RNA seq), cell culture, immune functional assays utilizing patient samples, ELISA assays, and flow cytometry (FACS). Expertise in molecular biology (PCR, RT-PCR, RNA seq), cell culture, immune functional assays utilizing patient samples, ELISA assays, and flow cytometry (FACS.
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RNA isolation and analysis by qRT-PCR, molecular biology (PCR, genotyping, cloning plasmid constructs, DNA isolation and sequencing analysis, CRISPR). With respect to laboratory technical skills, the individual must be proficient in cell/tissue culture, flow cytometry/sorting (FACS), protein isolation and analysis (western blot, ELISA, and.
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Molecular Biology: DNA, RNA, and protein extraction; cloning; PCR; qRT-PCR; recombinant virus production and purification. Immunology: ELISA; Cytokine assay; Flow cytometry. The Research Specialist will actively participate in biobanking activities and contribute to collaborative translational research in transplant immunology by using techniques of cellular and molecular biology, biochemistry, immunology, and proteomics, as well as maintaining the day-to-day needs of the lab.
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Design and execute lab experiments such as ELISA, in situ hybridization, DNA/RNA extraction, cDNA synthesis, ddPCR and blood processing. Design and execute lab experiments such as ELISA, in situ hybridization, DNA/RNA extraction, cDNA synthesis, ddPCR and blood processing.
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