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Technical requirements for this position include first-hand experience in the analysis of large-scale biological data, proficiency in cell and tissue culture, molecular biology skills, and an interest in working with transgenic animals and disease models.
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The Medical Research Specialist is responsible for performing highly specialized virology and immunology research pertinent to various TNPRC AIDS vaccine research projects, which includes conducting a wide range of molecular, virological, immunological and cell culture techniques and laboratory assays.
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In addition to being well versed with tissue engineering techniques, in vitro cell culture and animal models, strong preference will be given to applicants with backgrounds in vascular and/or neurobiology.
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Preferred skills include Cell culture, Virus culture, Bacteriology, Protein purification and conjugation, Microscopy, experience with Laboratory equipment including Spectrophotometer pH meter, centrifuges, column chromatography, and performing diagnostic assays.
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Experience in assay development, mammalian cell culture, ELISA, Western blotting, qPCR, and experience in standard molecular biology techniques such as cloning, protein expression and purification is preferred.
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3-5 years of experience performing cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay.
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The Biologics Virus Clearance Validation Leader is responsible for cell culture and purification process stretching studies, resin lifetime studies, buffer/solution hold time studies, at-scale membrane and resin lifetime verification studies, virus clearance studies, limit of in-vitro cell age studies, preparation for the regulatory files, PAI and support through the product lifecycle including knowledge management.
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Experience with mammalian cell culture, and in standard molecular biology techniques such as cloning, DNA purification, and protein expression is preferred. Execute in vitro analytical assays to evaluate samples with minimal supervision.
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The candidate must have the background and willing to learn new or expand upon existing skills involving cell culture, molecular biology, immunology, and biochemistry. Must have some laboratory experience working with molecular biology , cell culture or biochemical techniques.
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Essential skills includes: RT-qPCR, ELISA, Western blot, cell culture, necropsy. · Perform preclinical candidate screening of siRNAs leading to lead compound selection via combined in vitro and in vivo assays, working with or supported by cross-disciplinary groups, including chemistry, bioassay, histology, and in vivo pharmacology, and/or CROs.
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Under minimal supervision, s/he is expected to maintain and carry out mouse husbandry, perform in vivo experiments including mouse lung injury models and sample collections, and in vitro experiments including molecular sub-cloning, cell culture, flow cytometry, mRNA analysis, protein analysis and biochemical studies.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Our research utilizes in vivo methods, including genetic and surgical mouse models, and in vitro models such as intestinal organoids. Interested candidates with candidate should have a Ph. D. or MD/Ph. D., in relevant fields (ideally within the last three years) with extensive experience in cell/molecular biology, and metabolism.
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Memphis boats attractions, including Elvis Presley's Graceland, the Memphis Grizzlies, historic Beale Street, the National Civil Rights Museum, the second-largest urban county park in the United States, and the Memphis in May World Championship Barbecue Cooking Contest.
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Position requires 2 years of related experience, which must include some experience in each of the following skills: Proficiency in cell culture; Performing in vitro assays; General laboratory techniques (accurate use of pipettes, performing calculations involving molarity, % solutions, serial dilutions, and handling of biological matrices); General understanding of laboratory instrument use; and Competency with data analysis and interpretation.
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