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Develop in vitro cell-based assays (cell culture, gene editing, flow cytometry, high content imaging, cytokine profiling, cytotoxicity, proliferation, etc.) Experience with multi-color flow cytometry, panel design, primary cell culture, cell sorting and/or data analysis is preferred.
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The successful candidate will be expected to contribute to ongoing work in these areas, with specific duties including CRISPR gene engineering, pooled genetic perturbation screens, T cell culture, mouse handling and tumor models, FACS analysis, and a variety of genomics techniques.
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Minimum Field of Expertise: Cell and molecular biology; in vitro assays. Establish and maintain standard operating procedures (SOPs) for 2D and 3D cell culture workflows, including tissue processing, characterization, cell isolation, and maintenance.
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Experience in standard cellular and molecular biology techniques (Cell culture, ELISA, Cell-based assays, Immunofluorescence, Western blotting, Microscopy, RNA/DNA isolation, qPCR.
$65,000 - $78,000Full-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Support troubleshooting and optimization of cell culture and bioassays such as in-vitro potency. The successful candidate will have hands-on experience with cell-based in-vitro potency assays, SDS-PAGE, Automated Western blot assays, enzyme activity measurements, protein extraction, purification, and quantification methods.
$72,000 - $90,000Full-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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3-5 years of experience performing cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay.
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A strong understanding of cell culture methods - Potency, Agarose gel electrophoresis, SDS-PAGE, and cell banking is necessary. Support method development, transfer and qualification/validation activities associated with the cell-based potency assays for AAV-based gene therapy products.
$72,000 - $90,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Experience with cell culture and in vitro differentiation techniques. The lab studies adipose tissue, muscle and liver biology and tissue secreted exosomal miRNAs using in vivo and in vitro models, including differentiated human iPS cells.
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Work with the team to select indications, design, plan, and execute in vitro and in vivo experiments to select constructs, establish dose response relationships in animal models and cell culture systems.
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Performing cell culture and conducting in-vitro studies. EXPERIENCE AND TRAINING:Master's degree in Biology, Molecular Biology or similar discipline and at least one (1) year training in tissue engineering, drug-delivery, molecular biology, in vitro cell studies, in vivo animal studies, and related laboratory experience.
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Plan and execute studies requiring molecular biology techniques including E. coli cell culture, plasmid preparation, RNA synthesis, and purification with the aim to develop the processes that can be scaled up and transferred to MSAT labs.
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Perform mammalian tissue culture on mouse and human cell lines as well as primary immune cells. Primary responsibility for all aspects of mammalian cell culture, including media and buffer preparation, culture initiation, maintaining aseptic technique, propagation, and cryopreservation of cells, including primary human cells from commercial and/or patient sources.
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Lab work will involve tissue culture; patient tissue handling (cryo-preserving, isolating primary cells, developing organoids and PDXs); DNA, RNA, and protein-related assays; cell growth and death assays; animal work (xenograft tumor models, PDX, breeding.
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Perform cell culture experiments, RNA isolation, and in vitro assays. 2+ years’ experience with in vitro experiments, specifically mammalian cell culture. The incubent will work alongside subject matter experts in the fields of cell biology and biomedical engineering, and perform functional and targeted assays to describe the neurobiology of the model systems, such as RNA isolation/sequencing, ELISA, and other in vitro assays.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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