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Job Title: Postdoctoral Fellow Location: Cambridge, MA Duration: 12 months on Contract Description: Highlights: Strong Molecular Biology Description The Biological Process Development recently has Cell Line Development (CLD) group is responsible for producing cell lines that express clinical grade therapeutic proteins and viral vectors for Cell and Gene Therapies.
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Familiarity with cell culture processes and cell culture processes equipment, such as bioreactors, and downstream equipment for protein purification such as AKTA Pure/Avant is desirable.
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The position will require a high degree of autonomy in the discipline and work with colleagues in cell culture, bacteriology, virology, formulation, and bioanalytical science. Support cell culture and purification drug substance processes.
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Feeds cultures to maintain viability, passages cell lines and splits culture populations, prepares culture populations for long-term storage in liquid nitrogen, administers experimental treatments to culture environments, and measures responsive elements, as appropriate.
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2 years of experience understanding genetic mutations and their impact on the biological functionality of proteins, experience with genetic medicines including gene-therapy, gene-editing, cell therapy, mRNA and RNA silencing experience with pharmacokinetic, pharmacodynamics data and safety data.
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Utilize basic molecular biological techniques (, designing gene constructs, cloning, PCR, Western blot, gene transfection, mutagenesis, plasmid amplification, cell culture). This interdisciplinary lab employs various approaches including molecular biology, synthetic biology, imaging, microfluidics, biophysics, and mathematical modeling.
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Tissue culture /research in a GMP environment or blood banking and/or Hematology laboratory experience. Preferentially, this should include either tissue culture /research in a GMP environment or blood banking and/or Hematology laboratory experience.
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Details can be immunology/cell biology related various biological assays, analyzing patients’ samples and tumor-specific effector cells function, molecular biology related to cloning, DNA/RNA extract, and transduction to lentivirus or retrovirus vectors, and assisting with other lab tasks as needed.
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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Bachelor’s or master’s degree in Biological Sciences (Cell/Molecular Biology, Neuroscience, Microbiology, Immunology. Perform cell culture experiments, RNA isolation, and in vitro assays.
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Skills: cGMP, aseptic techniques, cell culture basics, cleanroom behavior, cell therapy, Biopharmaceuticals, training curricula design, hands-on training. Trainings are not limited to aseptic processing and qualification, cell culture technique, regulatory requirements such as GMP Documentation, cleanroom behavior, cGMP/ISO, and laboratory safety.
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In depth knowledge of cell culture and purification processes/ equipment. The role of the downstream bioprocess scientist will provide technical support for complex equipment and Biological and Biopharmaceutical processes.
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Applicants for postdoc should possess a Ph. D. in microbiology, microbial ecology, molecular biology, cell biology, biochemistry, genetics, bioinformatics, or a related field. A variety of approaches ranging from traditional oceanography to lab-based culture physiology work and genetics, genomics and targeted metagenomics are being used to investigate these microbes, their ecological roles and interactions.
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BS or MSc in Biological or Biochemical sciences, and 3-5 years relevant laboratory experience in antibody/protein characterization or quality control – particularly in the areas of immunology, molecular and cell biology, and protein chemistry.
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Design and execute cell-based studies to support identification, validation, and differentiation of small molecule protein degraders, implementing a wide variety of laboratory techniques including mammalian cell culture, Western blotting, CRISPR, RNAi, phenotypic assays, flow cytometry, etc.
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