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Good understanding of cell culture, molecular genetics, next generation sequencing techniques. Good understanding of cell culture and molecular genetics techniques. Good understanding of cell culture and molecular genetics technique.
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Will also work in tissue culture and multi-omics studies to elucidate the biological mechanisms of the origin of pre-cancerous lesions. S in biology, biomedical/biological engineering, chemical engineering, or related field; and work/internship experience in a wet lab, either in an academic research university or in a hospital/pharma industry setting.
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PREFERRED: master’s degree; and experience with biochemical assays of hepatocyte function, basic biology laboratory methods (e.g., aseptic technique, mammalian cell culture, immunoassays, DNA and RNA isolation, colorimetric plate readers, microscopy, ELISAs, spectrophotometers), and other standard analytical equipment.
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Proficiency in mammalian cell culture techniques, flow cytometry, qPCR, DNA and RNA extraction, transfection, ELISA, and western blotting. Investigate the biological impact of non-vial delivery of gene writer systems *in vivo.
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Working under the general supervision of a Senior Technical Research Assistant, Manager or Principal Investigator and following established policies and procedures, conducts a variety of specialized experimental procedures in human iPS cell culture and differentiation, Human fibroblast culture, fluorescence microscopy, FACS analysis, cell biology assays and molecular biology techniques.
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Tissue culture techniques and functional cell-based assay development. Biologics Engineering (BE) is large global organization with teams in Cambridge, UK and Gaithersburg MD responsible for the discovery and optimization of next generation biologics to create biological drug candidates for all key therapeutic areas such as oncology, respiratory and inflammation, cardiovascular, renal and metabolic diseases.
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Job Title: Postdoctoral Fellow Location: Cambridge, MA Duration: 12 months on Contract Description: Highlights: Strong Molecular Biology Description The Biological Process Development recently has Cell Line Development (CLD) group is responsible for producing cell lines that express clinical grade therapeutic proteins and viral vectors for Cell and Gene Therapies.
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Bachelor’s degree in biological science(s) field or related engineering with: For BMA III’s, 3-6years of experience in cGMP biotech manufacturing, preferably cell and gene therapies. Advanced understanding of primary cell culture and experience with equipment such as CliniMACS Plus, CliniMACS Prodigy, LOVO, bioreactors (single-use and fixed-bed), chromatography skids, TFF (hollow-fiber and flat-sheet) and WAVE bioreactors such as Xuri, G-Rex system.
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Details can be immunology/cell biology related various biological assays, analyzing patients’ samples and tumor-specific effector cells function, molecular biology related to cloning, DNA/RNA extract, and transduction to lentivirus or retrovirus vectors, and assisting with other lab tasks as needed.
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Design and execute cell-based studies to support identification, validation, and differentiation of small molecule protein degraders, implementing a wide variety of laboratory techniques including mammalian cell culture, Western blotting, CRISPR, RNAi, phenotypic assays, flow cytometry, etc.
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Execute assays to assess gene and protein expression (e.g., qPCR, ELISA, Flow Cytometry, Fluorescence Microscopy), and biological effects (e.g., function, viability, enzymatic activity) in cell lines and primary and iPSC derived 3D organoid cultures.
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BS in a biological science with at least 3 years of hands-on laboratory experience in cell or molecular biology is required. Perform cell culture (iPSCs, primary cells, immortalized cell lines) to support the research needs of the group.
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This is a contract role located in Boston, MA.Skills & Requirements:Experience in tissue culture and purification/quantification of nucleic acids/protein, including qPCR and ELISABS in a biological science with at least 3 years of hands-on laboratory experience in cell or molecular biology is requiredAt least 2 years of industrial experience Experienced with laboratory automation including liquid handling systems such as Perkin Elmer JANUS, HighRes liquid handling, or other systems.
Starting at $40 an hour (competitive)ExpandApply NowActive JobUpdated 3 days ago - UpvoteDownvoteShare Job
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Perform in vitro, ex vivo and in vivo testing of biomaterials (cell and tissue culture, physical characterization, biocompatibility tests, immunostaining, ELISA, and imaging). Support development and testing of molecular biology assays, such as qPCR, RT-PCR, gel electrophoresis, immunohistochemistry, and cell culture.
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In addition to basic methods of cell and molecular biology including signal transduction pathways, microRNA and long noncoding RNA, transgenics and gene knock out the Chen Lab has used a number of rodent animal models, in which stem cells, growth factors and advanced matrix scaffolds are applied.
$47,800 - $71,900 a yearFull-timeExpandApply NowActive JobUpdated Today
cell culture biological jobs in Watertown, MA
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