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Extensive hands-on experience with standard immunological techniques including multi-parameter flow cytometry, cell sorting, immunoassays such as ELISA, Cytotoxicity, in vitro co-culture assays (MLR, PBMC cultures, etc), and reporter bioassay development.
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Developing and optimizing project specific mechanistically relevant short term pathway activation as well as disease mouse models of disease - subsequent analysis of PK/PD and associated disease-related pathologies by various techniques such as flow cytometry, histology, protein multiplex ELISAs and focused or full transcriptome / proteome analysis.
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Proficient in industry-standard cell-based assay and bioanalytical techniques including TR-FRET, ELISA, flow cytometry, and reporter gene; experience with cellular/high content imaging and image analysis highly desirable.
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Experience in flow cytometry, as well as molecular and cellular biology; Collaborate with CRO and academic partners; employ cutting-edge in vitro and in vivo animal and human models to advance drug discovery and development programs up to and including IND filing.
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We are looking for a highly motivated, experienced cell biologist and assay development/screening scientist to join our growing team. Collaborate cross-functionally with Chemistry, Platform Development and In vivo biology teams to enable novel methods, assays and screening workflows.
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Excellent skills with cell culture such as primary cell culture, stable and transient protein expression, fixed and live cell imaging by IHC or immunofluorescence, flow cytometry. Collaborate with Protein Sciences to optimize the developability profiles of candidate molecules, and with In Vivo Pharmacology team to evaluate PK/PD, efficacy, and toxicity.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Experience developing and optimizing cellular assays (examples: MSD/Luminex/ELISA, multicolor flow cytometry) and/or molecular assays (examples: qPCR/PCR/ddPCR) is preferred. Here at Kelonia Therapeutics, we aren’t just a preclinical company; we’re trailblazers in viral engineering, pushing the boundaries of in vivo lentiviral gene and cell therapy.
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Perform experiments involving the in vitro screening and characterization of antibody and biologics-based drug candidates using various immunological, biochemical, and cell-based assays (e.g., ELISA, MSD, BLI, flow cytometry.
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Demonstrated experience in diverse molecular and biochemical laboratory techniques applied to cell characterization at molecular or genetic levels (immunofluorescence, flow cytometry, qPCR, RNA sequencing)Well organized, self-motivated, team oriented, ability to multitask with attention to detailExcellent communication and writing skillsProficient in computational analysis and coding pipelines (R Studio, Python, etc.
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Hands-on experience in multicolor flow cytometry and other routine immunological assays. The position involves collaboration with protein science, in-vivo biology, single-cell sequencing, bioinformatics, translational and clinical teams to execute and advance HiFiBiO’s drug discovery and translational research.
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Experience with modern cell and molecular techniques including culture of primary cells, cell-based assays, 3D and/or organoid culture; qPCR, western/ELISA/MSD, ChiP, FACS/flow cytometry, IF/IHC, microscopy.
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Proficiency in mammalian cell culture techniques, flow cytometry, qPCR, DNA and RNA extraction, transfection, ELISA, and western blotting. The Senior Research Associate/Associate Scientist will be a key contributor in our research efforts in delivering our novel gene writing technologies to target tissues *in vitro.
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Execute in vitro and ex vivo nonclinical experiments for the development of genetic medicines for the inner ear, including cell culture transfection/transduction, cochlear explant generation and analysis, and various molecular readouts such as flow cytometry, ddPCR/qPCR, and western blot.
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Enthusiastic about playing a pivotal role in the dynamic and multidisciplinary environment of a small biotech, specifically within the realm of in vivo sciences. The role will focus on cell-based and ex vivo approaches to design and understand biologic therapeutics, with the aim of nominating development candidates, and then supporting molecules through to IND. The grade for this position will be determined based on the candidate's level of experience.
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vivo flow cytometry scientist jobs in Waltham, MA
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