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Hands-on experience with cell-based assays and general molecular biology methods including, but not limited to, cell transfection, IVT, RNA extraction, qPCR, ddPCR, FACS, TapeStation, or Fragment Analyzer.
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Experience in cell biology, biochemistry, and/or molecular biology, in particular assay development and execution, is required. Experience with cell culture and in vitro assays is desirable.
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The Research Associate I/II will be responsible for tissue culture and *in vitro. Familiarity with molecular biology and biochemical assays such as: ELISAs, luciferase or fluorescent protein reporter assays, and Western blotting.
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The position involves mammalian cell culture, flow cytometry, molecular biology (plasmid construction, CRISPR gene editing), viral transduction to generate gene modified immune effector cells. The lab takes projects from conception to transgene design /genetic engineering, through reduction to practice, in vitro and in vivo studies, and ultimately translation to the clinic.
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Working independently, under the general supervision of senior personnel, the Research Associate will perform highly specialized experimental procedures involving in vitro protocols, advanced molecular genetics, and cellular biology techniques.
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Experience with standard molecular biology techniques such as PCR, bacterial plasmid production, in vitro transcription. Perform experiments to produce DNA templates for in-vitro transcription.
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Demonstrated experience in design and interpretation of in vivo and/or in vitro biological experiments. The candidate should have a solid foundation in applied statistics, deep generative models, computational biology, molecular biology paired with a strong work ethic and the ability to work independently and in highly matrixed teams.
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Develop, perform, interpret, and troubleshoot a broad range of in vitro assays including immune cell profiling, cytokine profiling, functional assays using primary cells, as well as cytotoxicity/apoptosis assays using primary and/or cultured cells.
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Computational analysis of large, complex, single cell genomics datasets from in vitro cellular model systems. Design and analyze bulk and single cell genomics datasets generated from our in vitro cellular and tissue model systems.
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Established background in cellular and molecular biology and competency with in vitro techniques is preferred. Design and conduct experiments to support new target validation, analysis and interpretation of results, with focus on in vitro and retinal in vivo assays/models to support discovery and translational drug development programs.
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Candidates should have basic training in molecular and cellular biology and biochemistry techniques, and experience in designing and executing in vitro and in vivo experiments. Candidates with experience performing bacterial culture for protein expression and cloning, protein purification using chromatography systems, protein characterization using SDS - PAGE , UV-Vis., and fluorescence spectroscopy, as well as running in vitro and cellular assays to evaluate the activity of proteins will be preferred in the selection process.
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Job Description: BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA. Qualified candidate will play an essential role in a high-energy DNA production and protein expression research laboratory.
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Experience with cell culture and interest in in vitro assay development. Ph. D. in molecular biology, cell biology, virology, genetics, or a similar field with 5+ years of industry or postdoctoral experience.
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They will be responsible for in vitro transcription (IVT) optimization, purification, and cell-based characterization of new RNA designs across various cell models. Hands-on experience with cell-based assays and general molecular biology methods including, but not limited to, cell transfection, IVT, RNA extraction, qPCR, FACS.
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This is a contract role located in Boston, MA.Skills & Requirements:Experience in tissue culture and purification/quantification of nucleic acids/protein, including qPCR and ELISABS in a biological science with at least 3 years of hands-on laboratory experience in cell or molecular biology is requiredAt least 2 years of industrial experience Experienced with laboratory automation including liquid handling systems such as Perkin Elmer JANUS, HighRes liquid handling, or other systems.
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vitro molecular jobs in Newton, MA
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