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As a Scientist on the in vitro Pharmacology team, you will be an integral part of our research and development efforts, leading bioanalytical support functions and assay development. Lead bioanalytical approach to support in vitro and in vivo studies assessing the efficacy, safety, and mechanism of action of gene therapies.
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Manifold Bio is a biotech company pursuing a pipeline of protein therapeutics using novel molecular measurement technologies and library-guided protein engineering. Manifold Bio is seeking a biologist enthusiastic about developing functional screens and in vitro mechanism-of-action assays, with an emphasis on neuroscience and neurodegenerative diseases.
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Other responsibilities will include routine molecular biology, histology/immunochemistry and flow cytometry in support of projects in cutaneous biology and immunology. We use a variety of in vivo and in vitro approaches to investigate a novel pathogenic link between VEGF-A and NLRP3 inflammasome activation.
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Experience in cell and molecular biology including mammalian cell culture, mRNA isolation/quantitation by qPCR, sequencing (NGS/Sanger), protein quantification by ELISA and/or western blot is required.
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Utilizing state-of-the-art single cell genomic technologies, high throughput screens, and chemical biology, our goal is to identify and validate novel molecular and immune targets using both in vitro and in vivo models of myeloma and its microenvironment.
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Deep hands-on experience with complex experimental design employing molecular biology techniques related to in vitro transcript analysis, gene expression, and RNA biology in mammalian cells.
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Experience with immunoassay techniques including IF, flow cytometry, and ELISA to support in vitro platform development studies. Masters or PhD in a related field (e.g. Cell Biology, molecular biology, etc.
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Preferred Qualifications: Molecular biology and protein biochemistry skills. Technical expertise with in vitro and ex vivo assays, mammalian tissue culture, reporter cell lines (including custom generation of reporter systems), and primary human cell cultures.
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The Senior Research Associate/Associate Scientist will be a key contributor in our research efforts in delivering our novel gene writing technologies to target tissues *in vitro. Familiarity with programs such as GraphPad Prism, FlowJo, and other molecular biology software/tools for data entry and analysis as well as well as PowerPoint for presentation.
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Expertise in molecular biology techniques, including in vitro transcription, qPCR, and ELISA. BS in Plant Science, Plant Pathology, Microbiology, Molecular Biology, or a related discipline.
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Working independently, under the general supervision of senior personnel, the Research Associate will perform highly specialized experimental procedures involving in vitro protocols, advanced molecular genetics, and cellular biology techniques.
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Works in unison with the development and research teams to evaluate DMPK properties of lead molecules as they relate to modes of administration and molecular design. A deep understanding of PK and ADA assay development principles and techniques (ELISA, MSD, BLI, SPR, MS, %recovery) with precision and accuracy.
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Interpret SAR and prioritize compounds for in vitro and in vivo biological and DMPK assessment. We are seeking a medicinal chemist to join the Molecular Discovery group. The successful chemist will help develop the molecular basis for our Encompass platform while interfacing with a team of scientists in mechanistic biology, biophysics and biochemistry, structural biology, computational, and translational sciences to bring forward new therapies.
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Hands-on experience with cell-based assays and general molecular biology methods including, but not limited to, cell transfection, IVT, RNA extraction, qPCR, ddPCR, FACS, TapeStation, or Fragment Analyzer.
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Experience performing in vitro nucleic acid-based techniques such as PCR, qPCR, gel electrophoresis, enzymatic treatment, and/or sequencing library prep, demonstrating a range of technical competencies.
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vitro molecular jobs in Cambridge, MA
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