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Experience with basic protein purification and characterization techniques (i.e., protein A and nickel capture, HPLC, SDS-PAGE, Endotoxin testing, etc.) Participate and assist in the daily activities of the purification group such as antibody and antigen purification, and characterization by various techniques including protein concentration determination, CE-SDS, SDS-PAGE, various HPLC methods, and endotoxin measurement.
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Solid working knowledge of various manufacturing platforms (Microbial fermentation process, IB recovery and refolding, CHO cell culture and purification, Pegylation, PEGylated protein purification etc.
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This work will require a number of biochemical and biologic techniques including (but not limited to): PCR; DNA and protein gel electrophoresis; Western blotting; standard cell culture; flow cytometry; transfections; DNA/RNA extractions; protein purification; buffer preparation; and microplate assays.
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Oligonucleotides to participate in the process design, development, and manufacture of RNAi therapeutic. (specializing in oligonucleotide synthesis, purification, and characterization.
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Interact with informatic engineers to develop processes that support automated antibody development workflows including antibody (DNA and protein sequence) registration, tracking, archiving, and inventory.
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5+ years of post-PhD experience in biotech industry, ideally in gene therapy or related next-generation drug development field such as biologics. Familiarity with protein engineering (involving model-guided approaches to protein design and molecular dynamic simulations.
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Oversee high-level strategic planning for developing bioinformatic analysis pipelines to assist pre-clinical therapeutic and platform teams, as well as to enable biomarker readouts for clinical trial development.
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Are excellent in most of the following: bacterial culturing, PCR, gel electrophoresis, molecular cloning, plate reader assays, cell-free expression systems, protein purification, buffer and media preparation, and DNA purification, sequencing, and analysis.
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I deal Qualifications B.S. or M.S. in biology, bioengineering, or another relevant field 3+ years of experience in a biotechnology company Experience with common molecular biology techniques: PCR, nucleic acid purification, and protein purification Experience with medium and high-throughput molecular biology workflows is a plus Experience with cell culture, microbes (e coli or yeast), or phage A working understanding of next-generation sequencing workflows.
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Ability to perform bacterial protein expression and purification and the analysis of ligand/protein interactions by fluorescence, isothermal titration calorimetry, or thermostability assays.
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Responsibilities -Develop assays for GMP release of gene therapy products-Develop assays for product characterization of gene therapy products-Develop assays for AAV/protein/DNA to assist in evaluation of process change, formulation andstability study-Report project status (development plans, timelines, and results) to supervisor and technical teams.
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Coordinate protein expression and purification with other teams at the firm. At least 2 years of research experience in protein biochemistry, protein engineering or related field.
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Reporting into a lead within the Process Sciences team, hiring an Analytical Development Associate Scientist within the Process Sciences Organization in Technical Operations. Key responsibilities of the Analytical Development Associate Scientist are summarized below, and these will evolve over time as the portfolio matures.
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As a Pharmacokinetics Scientist, you will utilize your experience to further characterize the novel kinetics of our new therapeutic modality and build complex models that inform study designs and decision-making throughout the drug development process.
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Coordinates with Cancer Immunology, Protein Chemistry, Antibody Engineering, and Translational Oncology groups to advance lead molecules of the projects. Supports biochemical and cellular characterization of protein therapeutics for immuno-oncology.
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process development protein purification jobs Company: Abbvie in South San Francisco, CA
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