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Familiarity with methods and systems used in biomanufacturing, essential molecular biology and methods such as PCR, purification, gel electrophoresis, sequencing, and high dimensional cell characterization approaches.
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The Research Associate will be responsible for conducting hands-on experiments including but not limited to molecular biology, mammalian cell culture, recombinant protein purification and characterization.
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Strong background in computational protein design, virology, molecular biology, and immunology is preferred. Significant expertise in protein biochemistry, including recombinant protein expression, purification and characterization is a plus.
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Strong experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots and other standard cell-based or biochemical assays.
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Hands-on experience with protein purification (HPLC, FPLC, SEC, IEX, IMAC, etc.) Bachelor or Master's degree in biology, biochemistry, molecular biology, or other related field. Hands-on experience with protein purification (HPLC, FPLC, SEC, IEX, IMAC, etc.
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Scientists in the institute’s five academic research departments work hand-in-hand with researchers of the Scripps Research Translational Institute and Calibr to merge foundational studies in biology, chemistry and computer science with translational science to produce pioneering drugs and advances in digital and precision medicine.
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Broad biochemistry technical knowledge, including but not limited to DNA manipulation, protein expression and purification, enzyme activity assays, and knowledge of cellular pathways. PhD + 2 years experience, OR MS + 8 years experience, OR BS + 10 years experience in Biology, Chemistry, Biochemistry or related disciplines.
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10+ years working in proteomics on protein extraction, separation, purification, and analysis techniques. Ph. D. in Molecular Biology, Biochemistry, Biophysics, Chemistry, Chemical Biology, or related field.
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Bachelors degree or Masters degree in (Bio)chemistry, Chemical Engineering, Microbiology, Biology or related field. Must have direct experience with oligo synthesis equipment (e.g., AKTA Oligopilot, AKTA Pilot/Beckman Purification systems, Dionex/Vanquish HPLC analytical systems, rotary evaporators.
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Basic tissue culture and purification responsibilities include performing cell culture work, antibody purification, conjugation, and associated quality control steps including purity analysis, isotype testing, mycoplasma detection, and specificity measurement by assigned methods.
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Performing DNbased labwork independently such as: aqueous chemical reactions on DNsmall molecule conjugates; DNA ligation or other molecular biology techniques; purification of DNA conjugates with High-Performance Liquid Chromatography (HPLC) or other techniques; analysis of DNsmall molecule conjugate compositions through mass spectrometry, electrophoresis, DNA quantitation and other relevant methods.
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Demonstrated expertise in molecular biology techniques, including DNA/RNA extraction & purification, NGS library preparation & QC, PCR, qPCR, etc. PhD or MS degree with 3-4+ years of professional experience in molecular biology, biotechnology, or biochemistry.
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Bachelor's Degree in life science field or related field preferably in Chemistry/Biochemistry/Biology/ Molecular Biology or equivalent experience. May perform enzymatic reactions, utilizing purification systems, authoring and implementing SOPs, and documenting action in batch records.
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The successful candidate will perform a variety of techniques, including cloning and expression of recombinant proteins, tissue culture, protein purification, and preparation for mass spectrometry analysis.
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Knowledge and experimental ability to perform procedures used in a molecular biology research laboratory setting such as sterile technique, DNA and RNA preparation, protein purification, PCR amplification, bacterial transformation, western blotting, cloning, bacterial growth and handling, plasmid preparation.
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purification biology jobs in San Diego, CA
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