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Assisting with molecular biology techniques such as cloning and plasmid purification, as well as isolation and analysis of RNA, DNA, and protein from mouse tissue and cellular samples. Assisting with biochemical techniques such as protein purification and labeling, protein-protein or protein-small molecule interactions, enzyme assays,and metabolite measurements.
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Viral DNA, RNA and protein extraction, purification, and analysis, including cloning, amplification and DNA sequencing. Viral DNA, RNA and protein extraction, purification, and analysis, including cloning, amplification and DNA sequencing.
$50,620 a yearExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Background in molecular biology techniques such as cloning, site-directed mutagenesis, DNA and RNA isolation and purification, sequencing, RT-PCR, and qPCR. Rescue and characterize pseudoviruses and/or RNA viruses.
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Under general supervision performs complex laboratory techniques including, but not limited to recombinant protein purification, DNA purification, flow cytometry. A special advantage of this rapid-freezing method is that we can trap and image dynamic states of functioning macromolecular assemblies, such as open and closed states of membrane channels and viruses actively transcribing RNA. Three-dimensional density maps are obtained by digital image processing of the high-resolution electron micrographs.
ExpandApply NowActive JobUpdated 6 days ago - UpvoteDownvoteShare Job
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Molecular experience in PCR, ddPCR, DNA/RNA extractions/purification is a plus. ATCC, a pioneer in biomaterials manufacturing, is seeking a Biologist to work onsite in the BioProduction Virology department in Manassas, VA. Prior experience with mamamalian cell culture and aseptic technique is required.
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
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Experience performing DNA, RNA, and protein preparation and purification is highly desired. Experience performing qPCR, qRT-PCR, DNA sequencing, ChIP-seq, mRNA isolation, northern blots, cDNA synthesis, immunoblots, co-immunoprecipitation, immunohistochemistry, and in situ hybridizations, are highly desired.
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Specific Responsibilities:Molecular Biology:DNA, RNA, Protein isolation, purification and expression Western blottingPCR, RT-PCRPlasmid preparationCloning, mapping and sequencing of DNA Recombinant virus production and purificationCellular Biology:Cell culture, cell isolation Functional studies TransfectionImmunologyAntibody production and purificationAdministrationMaintains detailed records of results which may include collecting, extracting & entering data.
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They will be responsible for in vitro transcription (IVT) optimization, purification, and cell-based characterization of new RNA designs across various cell models. Perform IVT & purification protocols for Client RNA therapeutic candidates.
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Support general lab activities such as: making solutions and buffers as needed and performing basic molecular cloning (primer design, PCR, DNA/RNA purification), as well as performing protein expression and protein purification and other molecular biology techniques.
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Hands-on expertise in routine lab techniques (PCR, DNA/RNA manipulation, purification and quantification techniques, etc.) Hands-on expertise in routine lab techniques (PCR, DNA/RNA manipulation, purification and quantification techniques, etc.
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Rescue and characterize RNA viruses. Bachelor's Degree in Virology, Molecular Biology, Immunology, or another related field with 8+ years of relevant industrial (preferred) or academic experience or a Master's Degree with some relevant experience.
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Perform experiments involving PCR, Quantitative PCR, Western Blots, PCR Arrays, RNA and DNA extraction, protein expression, and purification. The incumbent will perform experiments involving PCR, Real-time PCR, Northern and Western Blots, PCR Arrays, RNA and DNA extraction, protein expression, and purification.
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ATCC, a pioneer in biomaterials manufacturing, is seeking a Senior Biologist on-site 5 days/wk in Manassas, VA. A basic understanding of Virology and In vitro virus culture experience is a plus. Fitness and cell phone subsidies, and additional benefits such as Aflac, legal services, and pet insurance.
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Assisting with molecular biology and biochemistry techniques related to laboratory research projects such as PCR, molecular cloning, RNA/DNA isolation, and recombinant protein expression and purification.
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