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Performs routine techniques, such as primary cell culture, PCR, immunoblotting, immunohistochemistry, RNAScope in situ hybridization, fluorescence microscopy, etc. Performs routine techniques, such as primary cell culture, PCR, immunoblotting, immunohistochemistry, RNAScope in situ hybridization, fluorescence microscopy, etc.
$42,150 - $53,000 a yearFull-timeExpandApply NowActive JobUpdated 9 days ago - UpvoteDownvoteShare Job
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Function/Duties of Position Work on projects using techniques ranging from cell culture, western blotting, ELISA, 2-photon imaging, confocal microscopy, rodent surgery models, immunohistochemistry, and quantitative PCR. This includes data collection, optimization of experimental design in collaboration with other members of the team.
$61,318.4 - $97,968Full-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Additionally, bench-level experience in biochemical assays such as Western analysis, PCR and RT-PCR, immunohistochemistry, ELISA, luminescence, etc. Additionally, bench-level experience in biochemical assays such as Western analysis, PCR and RT-PCR, immunohistochemistry, ELISA, luminescence, etc.
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Responsible for developing the Solid Tumor Oncology Diagnostic Sciences strategy for in vitro diagnostic (IVD) programs for immunohistochemistry, Immunoassay, PCR and NGS (tissue and blood based) including bioinformatics approaches.
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Principal duties and responsibilities will include performing cutting edge, bench-level research in the following areas: molecular/cellular biology, human induced pluripotent stem cell (iPSCs) and neural stem cells, cellular differentiation, lineage phenotyping and basic neurobiology.
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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Work will involve, among other techniques, immunohistochemistry, imaging with confocal microscope, quantitative real-time PCR, Western blotting, DNA/RNA extraction, subcloning of plasmids, iPSC culture, iPSC transfection etc.
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Perform various experimental protocols involving cell culture, rodent surgeries, immunohistochemistry, microscopy, and molecular analysis relevant to the PI’s project in bone and neuroscience. Prior experience with sterile cell culture, mouse handling, microscopy, PCR and undergraduate research preferred, but not required.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linked immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Experience performing qPCR, qRT-PCR, DNA sequencing, ChIP-seq, mRNA isolation, northern blots, cDNA synthesis, immunoblots, co-immunoprecipitation, immunohistochemistry, and in situ hybridizations, are highly desired.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linker immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Modern research laboratories are equipped for tissue culture, real-time PCR, techniques for advanced protein detection, laser confocal microscopy, flow cytometry and molecular biology. Special diagnostic testing includes immunohistochemistry, in situ hybridization, advanced molecular diagnostics, electron microscopy, and whole slide imaging.
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Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to mammalian cell culture, DNA and RNA isolation, PCR, immunohistochemistry, DNA assembly, bacterial transformation.
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Key Responsibilities: 70% - Mammalian cell culture; RNA and DNA isolation; qRT-PCR, shRNA techniques; protein isolation and immunoprecipitation-western blotting; immunohistochemistry; in situ hybridization; fresh frozen and formalin-fixed paraffin-embedded tissue preparation and sectioning; and other histological/molecular/cellular biology techniques required for the research projects.
$42,088 - $53,536Full-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Immunohistochemistry and fluorescent microscopy. Performs molecular and protein analysis ( PCR , transfections, western analysis). Molecular Biology, Biology, Biochemistry, Physiology, Pharmacology, Microbiology, Immunology, other biomedical sciences.
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