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A minimum of 3 years of related work experience developing and performing cell or molecular assay techniques (e.g., qPCR, dPCR, cytokine analysis, ELISA, PBMC isolation, flow cytometry.
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Techniques used in the lab include, human primary cell cultures maintained in air-liquid interface cultures, fluorescence microscopy, flow cytometry, ELISA, molecular biology (DNA/RNA isolation, PCR), and viral replication to make recombinant viruses.
$55,000 - $65,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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A research-driven testing menu with quick result reporting allows the department to support clinical decisions in chemistry, immunochemistry, hematology, coagulation, anatomic pathology, cytology, microbiology, flow cytometry and molecular diagnostics for patients of all ages.
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In particular, the role requires carrying out all established workflows associated with hybridoma-based antibody discovery, including selection of rodents (wildtype and transgenic) as hosts for immunization; immunizations (protein, cell-based and genetic); evaluation of serum responses by ELISA; electro-cell fusion; hybridoma culture and screening; and VH-VL molecular cloning.
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Must have proficiency in a range of cell/developmental biology, molecular, immunological, and genomic techniques, including experience in tissue culture, flow cytometry, DNA methylation analysis, and chromatin immunoprecipitation.
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Facilities (mass spectrometry, cytometry, microscopy, biophysics and structural biology). molecular biology, protein chemistry, and fluorescence microscopy. Competent in molecular biology (cloning, and site-directed mutagenesis.
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Biochemistry and protein chemistry experience (flow cytometry, FACS, ELISA, western blotting, SDS-PAGE, etc.) Strong molecular biology background with a proficiency in DNA/RNA isolation and purification, cloning, sub-cloning, PCR, RT-qPCR, genotyping, expression construct design, transfection, CRISPR gene editing, and RNA profiling.
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Experience with a range of in vitro skills, e.g. cell culture, molecular biology, immunoblotting, flow cytometry and ELISA. Experience designing, conducting and analyzing multi-spectral flow cytometry immune-phenotyping studies.
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In vitro work includes a range of cellular assays cell culture including multiparametric flow cytometry and molecular biology techniques to help interpret in vivo observations. Bachelors Degree in Biochemistry Bioengineering Biology Cell Biology Immunology Molecular Biology or a related subject area and 4 years of scientific laboratory experience.
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Expertise in the following techniques is mandatory: magnetic cell isolation, cell culture, phenotyping of bone marrow progenitors using multicolor flow cytometry, CFU assays using CD34+ cells, ELISA, MSD, Western blot for signaling pathway proteins, basic molecular biology including isolation of nucleic acid and real-time PCR.
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Knowledge of and experience performing basic molecular biology techniques, including molecular cloning, nucleic acid isolation, PCR, and protein expression analyses. Extensive experience with flow cytometry assays.
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Lead the LGR Biology Team in collaboration with Technology and Computational Teams to develop state-of-the-art pooled and arrayed CRISPR screening platforms employing high-dimensional functional assays based on Perturb-seq, high-content cell imaging, flow cytometry and other omics technologies.
$112,500 - $176,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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1-3+ years of molecular biology wet lab experience (Western, PCR, ELISA, ELISpot, etc.) 2+ years of direct experience with multi-parameter flow cytometry. Experience with Next Generation Sequencing (NGS) technologies such as single-cell RNA seq.
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Analysis of flow cytometry results will be done using FlowJo or Cyflogic programs and involves analysis of fluorescence readouts, live/dead cells discrimination and multi color compensation. Advance skills in molecular biology methods, including cloning and PCR, tissue culture work with different cell lines, and transfection.
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Experience in molecular biology skills including western blotting, real-time qPCR, immunofluorescence, cell culture, mammalian overexpression systems, in vivo mouse models, and other techniques.
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