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Perform troubleshooting and evaluation of analytical methods such as molecular/ biological/ immuno-assays, including ddPCR, qPCR, ELISA, flow cytometry. Understanding of principles and practical applications of molecule-based assays, flow cytometry, cell enumeration and viability.
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Skilled scientist able to discern the advantages and fir fall of a range of analytical techniques such as molecular assays (ddPCR, qPCR, RT-qPCR), flow cytometry, ELISpot, viral assays, cytokine assays, ligand binding assays and Mass SpectrometryIn depth knowledge of concepts such of context of use of fit for purpose assay validation in bioanalysisOverseen assay transfer, scientific quality and study conduct at CRO partners.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linker immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Experience with in vitro mammalian cellular biology/immunology assays applied to physiologically relevant human cellular models, flow cytometry, multiplex cytokine measurements, cell activation, and cytotoxicity assays.
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Utilize a variety of techniques, including flow cytometry, molecular biology, and cellular assays, to evaluate the efficacy, safety, and pharmacokinetics of novel cell therapies. Examining, ensuring quality, and compiling data through immunoassay methodologies such as ELISAs, multiplex analysis, and flow cytometry, on samples obtained from clinical settings.
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The Research Associate II will be responsible for performing multi-color flow cytometry and troubleshooting immunoassays. Perform multi-color flow cytometry (up to 12 colors) and be responsible for flow cytometry panel design and troubleshooting.
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Strong hands-on skills in in vitro immunology, including immune cell isolation, culture and functional assays, basic biochemical and molecular techniques, including quantification of mRNA (qPCR) and protein expression, ELISA, Western Blotting, and Flow Cytometry assays.
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This drug discovery research position will support and collaborate with a research team to identify and validate disease-modifying molecular targets and support the progression of drug development projects to clinic.
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Hands on experience with various bioanalytical techniques including ELISA, qPCR and FACS/Flow Cytometry; B.S. in cell and molecular biology, immunology, biochemistry, or another Life Science field, with a master's degree preferred.
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Preferred: Interest in scientific sales and marketing, gaining entrepreneurship and business acumen skills, and prior advanced coursework in molecular or cell biology. Preferred: Aseptic technique and understanding of basic molecular biology techniques (qPCR, western blot, ELISA, Immunostaining) and mammalian cell culture.
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Performs molecular biology techniques. Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad.
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Biochemistry and protein chemistry experience (flow cytometry, FACS, ELISA, western blotting, SDS-PAGE, etc.) Strong molecular biology background with a proficiency in DNA/RNA isolation and purification, cloning, sub-cloning, PCR, RT-qPCR, genotyping, expression construct design, transfection, CRISPR gene editing, and RNA profiling.
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Experience with multicolor flow cytometry and ELISA. Experience with surface plasmon resonance (SPR) is a plus. Experience with multicolor flow cytometry and ELISA. Experience with surface plasmon resonance (SPR) is a plus.
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Perform molecular biology endpoints including qRT-PCR, ELISA, Western Blot, and Luminex. Proficiency with one or more additional molecular biology techniques including qRT-PCR, ELISA, Western Blot, and Luminex.
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Conduct a variety of specialized cellular and molecular assays, including but not limited to flow cytometry, luminescence assay, Western Blot, and cell culture, utilizing both mouse and human cells.
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cytometry molecular jobs in Rockville, MD
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