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Expertise in molecular studies techniques including DNA & RNA extraction & quantification, gel electrophoresis, western blotting, PCR and. Perform a wide range of sophisticated genetic, biological, and biochemical assays, including, but not limited to DNA transfection of cells, monolayer and anchorage-independent growth assays, immuno-precipitation assays, agarose gel electrophoresis, and in vivo and in vitro kinase assays.
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Essential Requirements Background in molecular biology techniques such as cloning, site-directed mutagenesis, DNA and RNA isolation and purification, sequencing, RT-PCR, and qPCR. Experience in cell culture, viral propagation, and viral titration.
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Experience running ELISA and PCR tests is essential; experience with related tests (e.g. Luminex IFA) would also be useful. Experience in ELISA and PCR assay development is highly desirable.
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As an Associate Scientist, you will use molecular biology, protein purification and characterization methods to support Discovery's protein engineering and technology development efforts. Must demonstrate proficiency with molecular biology methods, including primer design, PCR, gel electrophoresis, plasmid DNA preparation, cloning and sequence analysis using SnapGene or similar software.
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Knowledge of BioAnalytical Methods (PK, IM, LCMS, PCR) and interpretation of data. Knowledge of BioAnalytical Methods (PK, IM, LCMS, PCR) and interpretation of data. Maintain cell culture and perform immunocytochemistry; DNA isolation for qPCR. QC of BioAnalytical Data Packets, Protocols, reports, and data Tables (GCLP ad non-GXP.
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Experience in running batches for method development and validation of large molecules for PK, ADA and NAb using ELISA, MSD, Luminex, PCR and cell-based assays for e.g., proteins, peptides, monoclonal antibodies, oligonucleotides, cell and gene therapy products and other biomarker analysis including flow analysis.
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Background in molecular biology techniques such as cloning, site-directed mutagenesis, DNA and RNA isolation and purification, sequencing, RT-PCR, and qPCR. Background in molecular biology techniques such as cloning, site-directed mutagenesis, DNA and RNA isolation and purification, sequencing, RT-PCR, and qPCR.
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Strong molecular biology background with a proficiency in DNA/RNA isolation and purification, cloning, sub-cloning, PCR, RT-qPCR, genotyping, expression construct design, transfection, CRISPR gene editing, and RNA profiling.
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Skills:cell culture, ELISA, qPCR, PCR, GMP Experience:Entry Level1 year(s) responsibilities: As an Associate Scientist your activities will support assay development through commercial application of new and innovative service offerings within the biosafety testing space.
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Immediate need for a talented Assoc Scientist Bioscience. Understanding of DNA sequencing techniques (NGS and Sanger) and sequence analysis. Bachelor's Degree in virology, molecular biology, immunology, or another related field with 2+ years of relevant industrial (preferred) or academic experience or a Master's Degree with some relevant experience.
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Experience with Molecular Biology Techniques (Cloning, Mutagenesis, DNA/RNA Isolation/Purification, Sequencing, RT-PCR, qPCR, etc.) Associate Scientist - Virology. Immediate Opening for an Associate Scientist located in Gaithersburg, MD (Onsite.
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Experimental procedures include: molecular biology techniques such as bacteria cell cultures, isolation of DNA, gel electrophoresis, and PCR. Duties involve much micro pipetting and meticulous measuring.
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Excellent employment opportunity for an Assoc Scientist Bioscience in the Gaithersburg, MD area. A background in molecular/clinical virology and/or vaccinology is required. Assist in the preparation and review of bioanalytical data and reports suitable for regulatory submission.
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HJF is seeking a Postdoctoral Fellow Scientist to support the Research, Assessment and Development laboratory (RAD) in the Diagnostics and Countermeasures Branch (DCB), within the Center for Infectious Disease Research (CIDR) at the Walter Reed Army Institute of Research (WRAIR.
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Perform, with technical independence, basic and advanced laboratory procedures such as Plasmodium falciparum tissue culture, genetic manipulation, nucleic acid extraction, PCR, qPCR, CRISPR-targeting design, and next-generation RNA and DNA sequence analysis.
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pcr scientist jobs in Rockville, MD
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