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Ligland binding technology platforms (ELISA & MSD & Luminex)- Aseptic Cell Culture Techniques- Knowledge in basic Protein chaacterization (SDS Page Gel & Western Blots)- Expertise using SoftMac PRO and GraphPad Prism Software.
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Strong technical knowledge in Chemical, Biochemical, and Microbiological theory and methods such as HPLC, GC, SDS-PAGE, Protein Analysis, AAA, Animal-Based and Cell-Based Bioassays, and Sterility testing.
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Practices safety procedures at all times including Personal Protective Equipment (PPE), fire extinguishers, Safety Data Sheets (SDS), Lockout Tagout procedures, and infection control guidelines.
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Deka is currently seeking an Analytical Development Associate Scientist with experience in analytical methods, HPLC, cIEF, CE-SDS, potency assays. Provide lab-based assay development support for methods involving RP-HPLC, SEC-HPLC, CE-SDS, cIEF, ELISA, cell-based assays, gel electrophoresis, and western-blot.
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Performs cell-based bioassay methods and immunoassay methods, such as ELISA, SDS-PAGE, Westerns, qPCR, cIEF, and Biacore to test biopharmaceutical raw materials, in process samples, and drug substance materials under cGMP conditions.
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Experience with one or more of the following analytical techniques: liquid chromatography (SEC, IEC), capillary electrophoresis (CGE, CE-SDS), capillary isoelectric focusing (cIEF) Support protein therapeutics characterization using various biochemical and biophysical techniques, especially mass spectrometry-based methods (for example, peptide mapping for PTM quantitation, disulfide bond mapping, and intact mass analysis.
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Use established SOPs to independently carry out protein expression and purification processes for downstream biochemical and biophysical analysis such as SDS and Blue native PAGE, DLS, UV-Vis spectroscopy, fluorescence imaging, and confocal LSM.
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Assists in ensuring the safety of all community residents being familiar with his/her role in carrying out the sites fire, safety and disaster plans and by being familiar with current Safety Data Sheets (SDS.
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Biochemistry and protein chemistry experience (flow cytometry, FACS, ELISA, western blotting, SDS-PAGE, etc.) Biochemistry and protein chemistry experience (flow cytometry, FACS, ELISA, western blotting, SDS-PAGE, etc.
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Harmonize the SDS database with the chemical inventory, ensure locations are updated, archive dormant hazardous material SDS, ensure the latest SDS version is available, and provide GHS Label information for all SDS.
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Review SDS for technical and environmental health and safety considerations. Hospital or compounding pharmacy experience is preferable. This description reflects management's assignment of essential functions, it does not proscribe or restrict the tasks that may be assigned.
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Experience in with characterization of recombinant proteins, including but not limited to ELISA, MSD, SDS-PAGE, protein quantitation, etc. Experience in with characterization of recombinant proteins, including but not limited to ELISA, MSD, SDS-PAGE, protein quantitation, etc.
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