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Strong hands-on experience in mammalian cell culture including expertise in aseptic technique, culture optimization, cell counting, cryopreservation, and validation methods. Proficiency in experimental design, data analysis, and a range of biology skill sets including in vitro cell assay development, plate-based assays, Western blotting, protein quantification, ELISA, MSD, transfections, lentiviral transductions, siRNA/shRNA/CRISPR, qPCR, and viability assays.
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Lab-based experience with aseptic processing, cell culture, cell therapy manufacturing and cell isolation. Advanced degree (BS, MS or PhD) in Chemical/Biochemical Engineering, Biochemistry, Immunology, or related degree with experience (8+ for BS, 5+ for MS, 2+ for PhD) in process development and manufacturing sciences in cell therapy, biotechnology, or pharmaceutical industry.
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Be a cell therapy technical expert for cGMP manufacturing and aseptic processing. The incumbent will be responsible for a manufacturing team performing routine cGMP manufacture of a diverse portfolio of autologous and allogenic cell therapies at our state-of-the-art BaseCamp facility and provide leadership across both cell therapy and viral vector manufacturing.
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About Tenet Massachusetts Facilities: Saint Vincent Hospital – Worcester, MA MetroWest Medical Center – Framingham, MA Leonard Morse Hospital – Natick, MA (part of MWMC) MA - SVH Tenet’s Saint Vincent Hospital has been providing high-quality health care to Worcester and the surrounding communities for more than 125 years.
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The ideal candidate should have relevant experience in cell therapy process development, technology transfer to GMP manufacturing and GMP principles related to cell therapy, and enjoy fast-paced, collaborative and vibrant startup culture.
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Expertise in aseptic cell culture technique is essential, and previous experience using Benchling as an electronic lab notebook platform. Perform a range of immunological assays, including multi-color flow cytometry, ELISA, functional assays, and cell-based reporter assays.
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Working both independently and collaboratively to support cutting-edge Oncology drug discovery biology programs. Develop and execute mechanistic in vitro cellular assays to characterize mechanism of action of Client compounds.
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Tasks to be completed may include fermentation, protein purification, solvent extractions, tissue culture, preparation of bulk solutions, aseptic fills of buffers, filling and labeling of vials under sterile and non-sterile conditions, routine buffer and media preparation, filtration, vial preparation and sterilization.
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Aseptic technique and culture of mammalian, insect, and/or yeast cells. Molecular cloning, including DNA/RNA extraction, PCR, and Sanger or Next Generation Sequencing. Visterra, Inc., needs a highly motivated Senior Research Associate with research experience in biochemistry, immunology and/or molecular biology to help further our antibody-based drug discovery programs.
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Review and approve the Validation Master Plan (VMP) for manufacturing process, aseptic process, and shipping validation, working cross-functionally with key stake holders and responsible functions to ensure the defined VMP activities are executed, maintained and all supporting documentation is complete and accurate.
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Basic molecular biology skills – Aseptic technique, precise pipetting of small volumes in the microliter range, DNA/RNA extraction, nucleic acid quantification, automated electrophoresis, PCR, NGS a plus.
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Technical skills: aseptic technique, cell counting, passaging, cryopreservation, transfection, Gibson assembly, PCR, DNA/RNA isolation, ddPCR. Technical skills: aseptic technique, cell counting, passaging, cryopreservation, transfection, Gibson assembly, PCR, DNA/RNA isolation, ddPCR.
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Able to perform advanced, intermediate, and basic technical skills (compound administration, blood/tissue collection, clinical observation, necropsy, aseptic technique, implants, suturing, staples, surgical models, etc.
$39.13 - $58.72 an hourFull-timeExpandApply NowActive JobUpdated 11 days ago - UpvoteDownvoteShare Job
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Operate general manufacturing equipment in strict accordance with SOPs to complete tasks as noted above as well as large scale bioreactor operations, critical small or large volume sterile fills, and aseptic manipulation of cell cultures.
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Participates in providing a safe, aseptic, and orderly environment for patients in all Cardiology areas as needed. Facilitates the efficiency of the Invasive Cardiology and Non-Invasive Cardiology by performing a variety of duties associated with the care of cardiac patients, as designated by the Cardiology Manager(s.
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aseptic job Company: Tenet Massachusetts in Weston, MA
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