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General knowledge on protein purification, protein conjugation, HPLC analysis, SDS-PAGE analysis and mass spectrometric analysis of protein. skills: HPLC, SOP, In Vitro, In Vivo, Protein Purification, GMP (Good Manufacturing Practice.
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The purification group is responsible for non-GMP protein- drug lead generation, supporting the various research units and their pre-clinical therapeutic in- vitro and in- vivo needs. Qualified candidate will play an essential role in a high-energy protein purification research laboratory within BioMedicine Design.
$39.44 - $45.77 an hourFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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The ideal candidate will have hands-on experience working with mammalian cell culture, protein expression, purification and analytical QC using diverse biochemical methods such as ELISA, SDS-PAGE, Western blotting.
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Hands-on experience with separation based analytical methods (U/HPLC, SEC-MALS, LC-MS/MS, CGE, SDS-PAGE/WB, etc), with BLI, SPR or other protein-protein interaction technologies, and particle analysis & thermal stability (DLS, DSF, DSC, etc) is preferred.
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Deep technical experience in multiple key technical aspects of immunoassay development; including but not limited to protein chemistry, antibody screening, antibody conjugation, assay diluent formulation; multiplexing; preanalytical sample processing; scale-up, assay optimization and troubleshooting; Real- Time PCR, immunoassays (immunoblotting and ELISAs), and gel-based assays (SDS-PAGE and CE-SDS); multivariate data analysis; analytical validation; clinical validation.
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Experience using analytical techniques such as SDS-PAGE, RP-HPLC, ion exchange and size exclusion chromatography, LC-MS, UV-vis to characterize protein ligands and conjugates, underscoring a well-rounded understanding of protein characterization methods.
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With analytical techniques, such as QPCR, PCR, SDS-PAGE, Western blotting and ELISA - Exp. with electronic laboratory notebook applications, such as Benchling - Basic knowledge and application of theories, principles and techniques in molecular biology and cell biology "Nice to Haves": - Experience in a QC/GMP testing environment a plus Duties/Responsibilities: - Operate and maintain standard laboratory equipment and department-specific equipment.
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Erformance of routine biological assays including qPCR, ddPCR, ELISA, SDS-PAGE, and agarose gel electrophoresis is. Candidates should be self-starters, comfortable with autonomy, and embrace opportunities to develop new genomic medicines as part of a dynamic team within Alexion.
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Primary Job Responsibilities:Generate, purify, and analyze new bioconjugates to be used in both in-vitro and in-vivo studies while following established protocols. Proficiency with biochemical and biophysical techniques like SPR, BLI, ELISA, DSF, DLS, etc., for protein ligand characterization, highlighting a comprehensive skillset in protein analysis.
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The purification group is responsible for non-GMP protein-drug lead generation, supporting the various Pharmaceutical research units and their pre-clinical therapeutic in-vitro and in-vivo needs. Our client, a world-leading Pharmaceutical Company in Cambridge, MA is currently looking for a Protein Purification Scientist I to join their expanding team.
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Experience with the p erformance of routine biological assays including qPCR, ddPCR, ELISA, SDS-PAGE, and agarose gel electrophoresis is desirable. This position will play a key role in Alexion’s Viral Vector Product Development analytical development team , interacting with internal teams with in the Genomic Medicine and Product Development Clinical Supply group s.
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Characterize the proteins with various analytical techniques, including SDS-PAGE, Western blot, HPLC-MALS, BLI/SPR, DSF, and other functional activity assay. B.S./M.S. in Biochemistry, Chemistry, Pharmaceutical Sciences, Chemical Engineering, Biomedical Engineering or related field with 2+ years of experience in a Pharmaceutical or Biotechnology company (in an experimental laboratory environment.
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Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, HPLC, ELISA and other biochemical/biophysical assays such as BLI and SPR to determine protein purity and function.
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The project may involve creating virus-like particles (VLPs) by transfecting cells with viral structural proteins, designing sophisticated genetic circuits and assays, collecting data using state-of-the-art techniques (including flow cytometry, RT-qPCR, next-generation sequencing, ELISA, SDS-Page, Western immunoblots, and other analytic technologies), and using computational skills for working with cross-functional teams for data analysis.
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Characterize recombinant antibodies/proteins using SDS-PAGE, protein quantitation, HPLC, ELISA and other biochemical assays to determine protein purity and function. Experience in analytical methods for assessing the quality and purity of antibodies, such as SDS-PAGE and analytical SEC using HPLC system.
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