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We utilize immunofluorescence microscopy, cell and molecular biology, DNA damage repair, flow cytometry, and various sequencing assays on cell lines and patient-derived organoids to study DNA replication and cell cycle dynamics in different disease or pre-cancerous settings.
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DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS.
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Experience with state-of-the-art AI & ML methods applied to biological sequence data (DNA, protein)Hands-on experience with public omics, protein and immune repertoire datasets such as PDB, OAS, SAbDab. Strong experience with scientific programming languages (Python, R) and data science libraries.
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Experiments include in vivo assays, DNA/RNA/protein isolation and quantification including molecular biology techniques such as Gel Electrophoresis, qPCR, Western blot, and ELISA, tissue culture, microscopy, and various immunological assays.
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Perform research requiring molecular biology techniques including DNA/RNA analysis, qPCR/ddPCR, and other plate-based assays. To accelerate and expand its efforts, CRISPR Therapeutics has established strategic partnerships with leading companies including Bayer and Vertex Pharmaceuticals.
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The teacher must be confident and comfortable in a wet lab space teaching students about the basics of laboratory research and current laboratory techniques such as gel electrophoresis, DNA extraction, PCR, and ELISA. In addition, the teacher will be responsible for ordering reagents and materials, preparing experimental protocols, and setting up the equipment for each session.
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Configuration of data management, visualisation, and analytical platforms in support of DnA service delivery including Azure Databricks / Delta Lake and Synapse, SQLServer, Analysis Services / PowerBI. Put in place automated processes to reduce effort for colleagues in setup and maintenance of data platforms.
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Perform molecular techniques including DNA/RNA isolation, PCR, qRT-PCR, gene expression analysis, cell line engineering and tissue culture as needed. Hands-on experience in tissue culture, plate-based assays, RNA isolation, gene expression analysis (qPCR), western blot, ELISA, multiplex protein analysis, flow cytometry preferred.
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Support the characterization of PBMCs and iPSC-derived cell populations using immunocytochemistry, flow cytometry, DNA/RNA isolation, PCR, RNseq and other molecular biology techniques. Support the characterization of PBMCs and iPSC-derived cell populations using immunocytochemistry, flow cytometry, DNA/RNA isolation, PCR, RNseq and other molecular biology techniques.
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Experience with genomic technologies in human cellular systems including RNAi and CRISPR. BS degree in molecular and cell biology or related disciplines such as immunology or biological engineering with 1-2+ years of wet lab experience in academia or industry.
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Twist Bioscience synthesizes genes from scratch, known as "writing" DNA. Just as children learn to both read and write, the next phase of development for the genomics revolution is the ability to write DNA.
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Such research will include: creation and studying mosquito cell diseases, mosquito cell culture, transfection and gene transfer in cultured cells, DNA and RNA preparation, microscopy analysis, protein isolation and analysis.
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Must have hands-on experience analyzing DNA/RNA by IP-RP, IEX, LC-fluor and multi-modal LC techniques. Hands-on experience in oligonucleotide lipid nanoparticle delivery, production and purification short and large oligonucleotides, protein engineering and process development are a plus.
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1-5 years of patent prosecution experience in a biotechnology practice, focused on antibodies, DNA or RNA therapeutics, chemical biology, and gene-editing. 1-5 years of patent prosecution experience in a biotechnology practice, focused on antibodies, DNA or RNA therapeutics, chemical biology, and gene-editing.
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Knowledge and experience working with DNA manipulation techniques, enzymatic reactions, and sequencing chemistry, protein experience is a plus. Masters' of Science Degree in Chemical, Physical or Biological Science or MBA and 8+ years of experience in a molecular clinical laboratory (CLIA/CAP), at least 2 of which have been spent working under the direction of a doctoral level supervisor.
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