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Perform in-vitro assays; including ELISA, ELISpot, Luminex, flow cytometry, and qPCR. They will also perform downstream in-vitro assays. Perform in-vitro assays; including ELISA, ELISpot, Luminex, flow cytometry, and qPCR.
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Experience with ELISA/ WB, IHC/ Immunofluorescence and microscopy is a plus. They will be responsible for in vitro transcription (IVT) optimization, purification, and cell-based characterization of new RNA designs across various cell models.
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Responsibilities will include development and in vitro evaluation of drug delivery formulations - namely small molecule therapeutics and mRNA nanomedicines - and devices (~50% effort), and support preclinical evaluation of technologies in small and large animal models (~50% effort.
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Proficiency in mammalian cell culture techniques, flow cytometry, qPCR, DNA and RNA extraction, transfection, ELISA, and western blotting. The Senior Research Associate/Associate Scientist will be a key contributor in our research efforts in delivering our novel gene writing technologies to target tissues *in vitro.
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Generate, purify, and analyze new bioconjugates to be used in both in-vitro and in-vivo studies while following established protocols. Proficiency with biochemical and biophysical techniques like SPR, BLI, ELISA, DSF, DLS, etc., for protein ligand characterization, highlighting a comprehensive skillset in protein analysis.
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Strong expertise in immunological techniques and assay design/ development, including flow cytometry, ELISA, electrochemiluminescent (ECL) assays, cell-based assays such as ELISPOT and/or ASC assays, and animal models.
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The Senior Research Associate will be a key player in driving genomic medicine pipeline project through target validation, candidate selection and IND-enabling phases using in vitro, in vivo and ex vivo studies.
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A deep understanding of PK and ADA assay development principles and techniques (ELISA, MSD, BLI, SPR, MS, %recovery) with precision and accuracy. A deep understanding of PK and ADA assay development principles and techniques (ELISA, MSD, BLI, SPR, MS, %recovery) with precision and accuracy.
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Perform relevant in-vitro and in-vivo work to evaluate efficacy of therapeutic nanoparticles. Set-up, develop and prepare routine and specialized theranostic nanoparticles for in-vitro and in-vivo experiments.
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Proficiency in a variety of bioanalytical techniques including, but not limited to, ELISA, MSD, HTRF, flow cytometry, qPCR, NGS, mass spectrometry, and cellular imaging techniques. Technical expertise with in vitro and ex vivo assays, mammalian tissue culture, reporter cell lines (including custom generation of reporter systems), and primary human cell cultures.
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Primary Job Responsibilities:Generate, purify, and analyze new bioconjugates to be used in both in-vitro and in-vivo studies while following established protocols. Experience using analytical techniques such as SDS-PAGE, RP-HPLC, ion exchange and size exclusion chromatography, LC-MS, UV-vis to characterize protein ligands and conjugates, underscoring a well-rounded understanding of protein characterization methods.
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In vitro phage library panning and clone screening via phage ELISA and periprep ELISA. High-throughput binding assays for IgG testing such as ELISA and similar techniques. In vitro phage library panning and clone screening via phage ELISA and periprep ELISA.
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The qualified candidate should have considerable experience in systematic or local injection, brain and tissue collection, molecular analysis, and downstream analysis (RT-qPCR, western blot, ELISA, bDNA quantification and IHC.
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Experience with molecular biology assays (ELISA, PCR, protein expression, phage display, next-gen sequencing, cell culture); Experience with molecular biology assays (ELISA, PCR, protein expression, phage display, next-gen sequencing, cell culture.
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Develop and optimize bioanalytical assays for the quantitative measurement of target proteins (ELISA, MSD). This is an exciting opportunity to apply your scientific skills to conduct state of the art experiments meant to identify and validate unique and novel targets in the areas of autoimmunity, chronic inflammatory diseases and cancer using in vitro and in vivo humanized experimental models.
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elisa vitro jobs in Lexington, MA
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