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At least 5+ years of hands-on industry and/or academic experience with next-generation DNA sequencing (NGS) and associated methodologies including DNseq and/or RNseq protocols. At least 5+ years of hands-on industry and/or academic experience with next-generation DNA sequencing (NGS) and associated methodologies including DNseq and/or RNseq protocols.
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Extracts DNA from peripheral blood or solid tissue (e.g., lymph node, spleen), performs DNA amplification, and determines HLA antigens by current methods. Isolates and separates lymphocytes from peripheral blood into two major subpopulations (B and T lymphocytes) for determination of HLA antigens via complement dependent cytotoxicity and/or extracts DNA from blood for HLA typing via molecular methodology.
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Experience with standard molecular biology techniques such as nucleic acid isolation, purification and quantitation, bioanalyzer, DNA/RNA quantitation, qPCR or Next Generation Sequencing. Experience with standard molecular biology techniques such as nucleic acid isolation, purification and quantitation, bioanalyzer, DNA/RNA quantitation, qPCR or Next Generation Sequencing.
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Perform molecular techniques including DNA/RNA isolation, PCR, qRT-PCR, gene expression analysis, cell line engineering and tissue culture as needed. MSD ELISA About PepGen PepGen Inc. is a clinical-stage biotechnology company advancing the next-generation of oligonucleotide therapies with the goal of transforming the treatment of severe neuromuscular and neurological diseases.
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Maintenance of Puigserver Lab animal (laboratory mouse) colony, including weaning, tail biopsies, DNA preparation and genotyping by PCR. Maintenance of Puigserver lab animal protocols. Assisting with biochemical techniques such as protein purification and quantification, enzyme assays and metabolite measurements.
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Under the general supervision of the investigator, performs complex laboratory experiments in the field of cell biology including: cell culture, ELISA, DNA/RNA isolation, PCR, general biochemistry, immunohistochemistry, flow cytometry, and microscopy.
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Basic wet lab skills, including but not limited to, molecular biology and biochemistry techniques such as DNA and RNA extraction, lysate preparation, qPCR, protein quantification, Western blot/WES/JESS, and ELISA is preferred.
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DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS.
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Bachelor's degree and one (1) year of previous HLA experience or molecular genetics laboratory experience, preferably including DNA extractions, PCR amplification, probe hybridization/detection or DNA sequencing.
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Screen offspring of several mouse strains by preparation of genomic DNA and PCR or by isolation of blood cells and flow cytometry. A Research Assistant is wanted to assist with ongoing experiments that include human beta cell culture, molecular biology techniques, flow cytometry, histology, mammalian cell culture and animal studies, and banking of human and mouse iPS cells.
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In addition, Fusion is pursuing combination programs between RCs and DNA Damage Response Inhibitors (DDRis) and immune-oncology agents. Fusion has a fully operational Good Manufacturing Practice (GMP) compliant state-of-the-art radiopharmaceutical manufacturing facility to meet supply demand for Fusion's growing pipeline of RCs.
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Prior hands-on experience with wet lab molecular biology techniques such as PCR/qPCR, DNA assembly methods, sequencing library is a plus, showcasing practical laboratory experience. We're innovating a cutting-edge DNA computing platform, and we are seeking a dynamic Software engineer with a background in synthetic or molecular biology to join our quest.
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Strong laboratory skills and a high degree of attention to detail with the experience in following molecular biology and analytical techniques: PCR, gel electrophoresis, gel imaging, SDS-PAGE Mammalian cell culture, transfection, transduction (AAV) Nucleic acid preparation (DNA, RNA), ddPCR, qPCR and data analysis.
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You have experience with cell line engineering and validation (e.g. stable retroviral transduction, transient DNA/mRNA transfection, CRISPR). The Team Alloy’s Translational Research team supports our drug discovery partners with in vitro and in vivo testing of therapeutic drug candidates across multiple modalities.
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The BEP production groups are looking for a laboratory technician to provide DNA, RNA and protein production support for the RNA and protein activities in BEP. The candidate is expected to have a background in biochemistry techniques including nucleic acids, protein purification, and cell culture.
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