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We utilize immunofluorescence microscopy, cell and molecular biology, DNA damage repair, flow cytometry, and various sequencing assays on cell lines and patient-derived organoids to study DNA replication and cell cycle dynamics in different disease or pre-cancerous settings.
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Must have hands-on experience analyzing DNA/RNA by IP-RP, IEX, LC-fluor and multi-modal LC techniques. Develop and implement methods intended for a variety of purposes, including release and stability testing, product characterization, and high throughput analysis.
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In addition, under supervision of the Principal Investigator, the Research Technician conducts a variety of experiments following established procedures and protocols, which may include genetic assays, PCR assays, creation of yeast mutants, and molecular biological research using DNA and protein detection techniques.
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DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS.
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Experience with state-of-the-art AI & ML methods applied to biological sequence data (DNA, protein)Hands-on experience with public omics, protein and immune repertoire datasets such as PDB, OAS, SAbDab. Strong experience with scientific programming languages (Python, R) and data science libraries.
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Experiments include in vivo assays, DNA/RNA/protein isolation and quantification including molecular biology techniques such as Gel Electrophoresis, qPCR, Western blot, and ELISA, tissue culture, microscopy, and various immunological assays.
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The Clinical Laboratory Technologist will be responsible for performing high-complexity molecular testing according to established SOPs, including DNA and RNA extractions, NGS library preparation, hybridization-based enrichments, and next-generation sequencing.
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The teacher must be confident and comfortable in a wet lab space teaching students about the basics of laboratory research and current laboratory techniques such as gel electrophoresis, DNA extraction, PCR, and ELISA. In addition, the teacher will be responsible for ordering reagents and materials, preparing experimental protocols, and setting up the equipment for each session.
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Such research will include: creation and studying mosquito cell diseases, mosquito cell culture, transfection and gene transfer in cultured cells, DNA and RNA preparation, microscopy analysis, protein isolation and analysis.
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Support the characterization of PBMCs and iPSC-derived cell populations using immunocytochemistry, flow cytometry, DNA/RNA isolation, PCR, RNseq and other molecular biology techniques.
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The successful candidate should be a self-starter who can perform wetland and other natural resources surveys, environmental permitting, and natural resources planning activities for a variety of projects including Transportation, Energy, Water/Wastewater and Stormwater Infrastructure Construction.
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Demonstrated ability to perform accurate data analysis and carefully maintain a notebook record of lab activities. Assist in developing, executing, and troubleshooting new cell-based assays, protocols, and analysis methods.
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Configuration of data management, visualisation, and analytical platforms in support of DnA service delivery including Azure Databricks / Delta Lake and Synapse, SQLServer, Analysis Services / PowerBI. Put in place automated processes to reduce effort for colleagues in setup and maintenance of data platforms.
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You have experience with cell line engineering and validation (e.g. stable retroviral transduction, transient DNA/mRNA transfection, CRISPR) Family planning support up to $45,000 (e.g., IVF/PGT, adoption, surrogacy, egg retrieval.
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Work with human cell lines, recombinant DNA, and common molecular biology reagents in a laboratory environment that may include hazardous chemicals and blood borne pathogens. Tissue culture experience is required—including strong aseptic technique and BSL2 stewardship, fixation and staining of cells, and troubleshooting.
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dna job Title: planning and analysis in Cambridge, MA
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