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Scientific training and hands-on experience with various cell-based and molecular assays, including but not limited to 2D and /3D cell culture, cell-based potency assay, ELISA, western, qPCR, RT-qPCR, and DNA/RNA/protein extraction from cells and animal (including NHP) tissues.
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Must have hands-on experience analyzing DNA/RNA by IP-RP, IEX, LC-fluor and multi-modal LC techniques. Hands-on experience in oligonucleotide lipid nanoparticle delivery, production and purification short and large oligonucleotides, protein engineering and process development are a plus.
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We utilize immunofluorescence microscopy, cell and molecular biology, DNA damage repair, flow cytometry, and various sequencing assays on cell lines and patient-derived organoids to study DNA replication and cell cycle dynamics in different disease or pre-cancerous settings.
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In addition, under supervision of the Principal Investigator, the Research Technician conducts a variety of experiments following established procedures and protocols, which may include genetic assays, PCR assays, creation of yeast mutants, and molecular biological research using DNA and protein detection techniques.
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DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS.
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Experience with state-of-the-art AI & ML methods applied to biological sequence data (DNA, protein)Hands-on experience with public omics, protein and immune repertoire datasets such as PDB, OAS, SAbDab. Strong experience with scientific programming languages (Python, R) and data science libraries.
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Experiments include in vivo assays, DNA/RNA/protein isolation and quantification including molecular biology techniques such as Gel Electrophoresis, qPCR, Western blot, and ELISA, tissue culture, microscopy, and various immunological assays.
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The Clinical Laboratory Technologist will be responsible for performing high-complexity molecular testing according to established SOPs, including DNA and RNA extractions, NGS library preparation, hybridization-based enrichments, and next-generation sequencing.
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In CMOS-bio interfaces, CMOS integrated circuits are interfaced with biological systems such as mammalian neuronal networks and DNA molecules for applications in neuroscience, neurotechnology, machine intelligence, and molecular data storage.
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The teacher must be confident and comfortable in a wet lab space teaching students about the basics of laboratory research and current laboratory techniques such as gel electrophoresis, DNA extraction, PCR, and ELISA. In addition, the teacher will be responsible for ordering reagents and materials, preparing experimental protocols, and setting up the equipment for each session.
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Such research will include: creation and studying mosquito cell diseases, mosquito cell culture, transfection and gene transfer in cultured cells, DNA and RNA preparation, microscopy analysis, protein isolation and analysis.
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Oversees the validation and integration of new diagnostic assays into the clinical flow cytometry laboratory framework, including measurable residual disease (MRD) monitoring, myelodysplastic syndrome (MDS) assessment, cell sorting for FISH and molecular applications, DNA ploidy, and flow cytometry-based functional assays.
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Support the characterization of PBMCs and iPSC-derived cell populations using immunocytochemistry, flow cytometry, DNA/RNA isolation, PCR, RNseq and other molecular biology techniques.
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The successful candidate should be a self-starter who can perform wetland and other natural resources surveys, environmental permitting, and natural resources planning activities for a variety of projects including Transportation, Energy, Water/Wastewater and Stormwater Infrastructure Construction.
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Experience with genomic technologies in human cellular systems including RNAi and CRISPR. BS degree in molecular and cell biology or related disciplines such as immunology or biological engineering with 1-2+ years of wet lab experience in academia or industry.
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dna job Title: planning and in Cambridge, MA
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