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Lead with hands-on support in commercial cell line development contract campaigns and single-cell assay development. Scientist/Senior Scientist, Cell Line Development. At OneCyte, you'll play a pivotal role in advancing biotechnology through commercial cell line development contract campaigns, cutting-edge R&D efforts, and the development of state-of-the-art technologies.
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Your hands on experience with cell-based assays (e.g., cell-binding, proliferation, cytotoxicity, apoptosis, receptor activation, internalization, cytokine secretion, immune cell activity, gene expression response) for antibody and biologics screening and characterization (reporter cells, primary cells) using a variety of techniques (e.g., flow cytometry, luminescence, fluorescence, cell imaging) is needed.
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Basic knowledge of cell culture manufacturing principles, risk assessments (FMEA), Chemistry, Manufacturing, and Controls (CMC) principles, and tech transfer. Execute experiments to support Cell Line Development and Scale up of PSC-derived cells.
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High level of expertise in stem cell biology, process engineering, and project management, ensuring the successful development and implementation of cell therapy manufacturing processes.
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Vertex currently operates at the forefront of rare disease scientific innovation and has successfully developed and commercialized multiple breakthrough medicines for Cystic Fibrosis (CF) and Sickle Cell Disease.
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POSITION SUMMARY: Provides services as a Public Safety Officer within BMC Department of Public Safety to provide police, security, parking, transportation and control center services as necessary.
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We are currently seeking a cell biologist/pharmacologist to join the CDoT Cell Pharmacology team. In this role, you will report to a research scientist and work across multi-disciplinary teams to drive the development and execution of novel cell-based assays to empower target validation, hit discovery and lead optimization.
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Experience with cell and gene therapy test methodologies (qPCR/ddPCR, Flow Cytometry, ELISA, and cell-based assays). The ElevateBio ecosystem combines multiple R&D technology platforms - including Life Edit, a next-generation, full-spectrum gene editing platform; comprehensive cell engineering technologies; and an expanded viral and non-viral therapeutic delivery platform - with BaseCamp®, its end-to-end genetic medicine cGMP manufacturing and process development business, to accelerate the discovery and development of advanced therapeutics.
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Proven track record of leading successful in vitro pharmacology efforts in the field of Cell Therapy, including assay development and validation, candidate screening, and mechanism of action studies.
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Job Title: Postdoctoral Fellow Location: Cambridge, MA Duration: 12 months on Contract Description: Highlights: Strong Molecular Biology Description The Biological Process Development recently has Cell Line Development (CLD) group is responsible for producing cell lines that express clinical grade therapeutic proteins and viral vectors for Cell and Gene Therapies.
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Demonstrated experience in diverse molecular and biochemical laboratory techniques applied to cell characterization at molecular or genetic levels (immunofluorescence, flow cytometry, qPCR, RNA sequencing)Well organized, self-motivated, team oriented, ability to multitask with attention to detailExcellent communication and writing skillsProficient in computational analysis and coding pipelines (R Studio, Python, etc.
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The company was founded by world leading CRISPR, gene editing, and molecular biology pioneers from MIT, Broad Institute, Harvard and Duke Universities and world-class investors using a novel targeted cell and gene therapy platform.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Perform cell culture, prepare cell slides or/and tissue sections, carry out immunofluorescence staining, microscopy, image processing, protein pull-down, protein measurement, and purification.
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Familiarity with IND-enabling studies and preparing materials for the pharmacology section of an IND.Excellent skills with cell culture such as primary cell culture, stable and transient protein expression, fixed and live cell imaging by IHC or immunofluorescence, flow cytometry.
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