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Scientific tasks include development and execution of experiments involving PCR, qPCR, in vitro fermentation, headspace gas chromatography, cell culture with rich or freshwater media, protein expression in bacteria, cell harvesting, protein characterization.
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Flask and bioreactor fermentation execution. Operate and maintain instrumentation for bacterial fermentation (BSL2) and primary recovery (filtration, etc.) Confidence working in biosafety cabinets while performing cell plating and inoculation of flasks/bioreactors.
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Extensive experience in microbial fermentation, protein engineering, and bioprocess design. Lead the development and optimization of precision fermentation processes, ensuring scalable, efficient, and high-quality alternative protein production with commercial viability.
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Job DescriptionSenior Associate Scientist, Cell Culture Process Development - Pre-Pivotal Biologics CMC Development – Foster City, CAAt Gilead, we’re creating a healthier world for all people. Job DescriptionThe Senior Associate Scientist, Cell Culture Process Development (Pre-Pivotal) will lead and execute cell culture process development laboratory experimentation for Gilead biologics entities during pre-pivotal phases development (pre-clinical, phase I/II.
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Skills: Molecular Biology, Cell Biology, Biology, Chemistry, Chromatography, Protein Purification, Cell Cultures, Manufacturing Operations. Display understanding of upstream and downstream process theory (i.e., Fermentation, Harvest, Tangential Flow Filtration, Column Chromatography) and equipment operation.
$20 - $31.1 an hourExpandApply NowActive JobUpdated 13 days ago - UpvoteDownvoteShare Job
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Solid working knowledge of various manufacturing platforms (Microbial fermentation process, IB recovery and refolding, CHO cell culture and purification, Pegylation, PEGylated protein purification etc.
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Reporting to the Vice President, CMC Operations, the Head of/Senior Director CMC Operations (Drug Substance) will manage activities related to Process Development, Tech Transfer and Manufacturing of Drug substance.
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Act as the in-house validation expert and be responsible for CMC process validation including risk assessment, IPC, process characterization and validation following QbD guideline, as well as successful execution of PPQs and PAI to support BLA/MAA approval.
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Proficient in mammalian cell culture, particularly stemming from work with iPSC and/or ESC. Additional skills may include one or more of the following: stem cell differentiation, high-throughput cell-based screening, genome editing, flow cytometry, advanced microscopy, cell line quality control.
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Industry experience in sterile fermentation techniques preferably mammalian cell culture. Prior experience in bioreactor operation and cell culture media preparation is a must. Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work and occasionally lifting 50Ibs.
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Take routine analytical measurements for cell growth, gas-content analysis, pH, osmolality, nutrient and metabolite measurements and HPLC analysis of monoclonal antibody titers. Perform routine tasks such as media/buffer preparation, thawing of cryopreserved cells, cell culture passaging, aseptic sampling and banking competently and independently to support ongoing projects in cell culture lab.
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Preferred State-of-the-art transient and stable protein expression experience Relevant bioreactor experience Hands-on experience with automation and liquid handlers like Tecan Freedom Evo and Hamilton Microlab Star Knowledge of cell biology, cell engineering, molecular biology, recombinant protein purification and protein characterization is preferred.
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Led by a management team with significant experience in cell therapy, Allogene is developing a pipeline of "off-the-shelf" CAR T cell product candidates with the goal of delivering readily available cell therapy on-demand, more reliably, and at greater scale to more patients.
$80,000 - $105,000 a yearExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Fermentation Process Development, Scientist II - Senior Scientist. Utilize diverse gene delivery techniques such as CRISPR KO/KI, lentivirus/gamma retrovirus transduction for cell engineering of primary T cells, iPSCs, and iPSC-derived T cells.
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BS/MS in Stem Cell Biology, Molecular Biology, Genetics, or a related disciplineStrong background in sterile aseptic mammalian cell culture techniques and experience working with human iPSCsTechnical expertise in at least two of the following areas: cell based functional assays, image acquisition/analysis, molecular assays (NGS, RT-PCR, dd-PCR)Proven ability to multitask, collaborate and communicate effectively.
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fermentation cell jobs in South San Francisco, CA
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